Xie Xiaofei, Zhang Ying, Teng Zhongqiu, Duan Biao, Hai Yan, Wang Mingliu, Shao Zhujun, Liang Weili, Kan Biao, Yin Feifei, Qin Aiping
Hainan Medical College, Haikou, Hainan Province, China.
State Key Laboratory of Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
China CDC Wkly. 2024 Jul 26;6(30):734-739. doi: 10.46234/ccdcw2024.165.
and -like diseases, resulting from obligate intracellular Gram-negative bacteria, pose a growing public health threat in China. To assess the current prevalence of these diseases on Hainan Island, a study was conducted on 9 bacterial pathogens found in patients with undifferentiated febrile illness (UFI) treated in Haikou between 2018 and 2021 using a TaqMan Polymerase Chain Reaction (TaqMan PCR) array.
Blood samples (=503) were collected from patients with UFI between 2018 and 2021. The samples were screened for spp., (), (), , , , spp., , and using a TaqMan PCR array. Positive samples (Ct<35) underwent confirmation through nested PCR, sequencing, and phylogenetic analysis.
and were detected in the patients at positive rates of 14.51% (73/503) and 5.57% (28/503), respectively. Co-infection of and was identified in scrub typhus (ST) positive populations from Hainan (10.96%, 8/73), Guangxi (61.54%, 8/13), and Yunnan (5.36%, 3/56) provincial-level administrative divisions (PLADs) of China.
An increased prevalence rate of ST and a decreased prevalence of rickettsioses were observed in patients with UFI in Hainan compared to a decade ago. The co-infection of and poses a current public health threat in China.
由专性细胞内革兰氏阴性菌引起的类疾病在中国对公共卫生构成了日益严重的威胁。为评估海南岛这些疾病的当前流行情况，于2018年至2021年期间，在海口对患有未分化发热性疾病（UFI）的患者中发现的9种细菌病原体进行了一项研究，采用TaqMan聚合酶链反应（TaqMan PCR）芯片技术。
在2018年至2021年期间收集了患有UFI患者的血样（n = 503）。使用TaqMan PCR芯片对样本进行筛查，检测恙虫病东方体、五日热巴通体、战壕热立克次体、普氏立克次体、莫氏立克次体、贝纳柯克斯体、嗜吞噬细胞无形体、人粒细胞埃立克体和查菲埃立克体。阳性样本（Ct<35）通过巢式PCR、测序和系统发育分析进行确认。
在患者中检测到恙虫病东方体和五日热巴通体，阳性率分别为14.51%（73/503）和5.57%（28/503）。在中国海南、广西和云南省级行政区（PLADs）的恙虫病（ST）阳性人群中，发现恙虫病东方体和五日热巴通体的共感染情况，分别为10.96%（8/73）、61.54%（8/13）和5.36%（3/56）。
与十年前相比，在海南患有UFI的患者中观察到恙虫病的患病率增加，立克次体病的患病率下降。恙虫病东方体和五日热巴通体的共感染目前对中国公共卫生构成威胁。
