Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Faculty of Biology, University of Freiburg, Freiburg, Germany.
Methods Mol Biol. 2024;2845:27-37. doi: 10.1007/978-1-0716-4067-8_3.
Synthetic tethering approaches induced by chemical means offer precise control over protein interactions in cells. They enable the manipulation of when, where, and how proteins interact, making it possible to study their functions, dynamics, and cellular consequences at a molecular level. These methods are versatile, reversible, and adaptable, allowing the dissection of complex cellular processes and the engineering of cellular functions. Here, we describe two chemically induced dimerization systems in the model organism Saccharomyces cerevisiae. Using the autophagy pathway as an example, we show how these approaches can be used to dissect molecular events in cells.
化学诱导的合成连接方法提供了对细胞中蛋白质相互作用的精确控制。它们能够控制蛋白质何时、何地以及如何相互作用，从而可以在分子水平上研究它们的功能、动态和细胞后果。这些方法具有多功能性、可逆性和可适应性，允许对复杂的细胞过程进行剖析和对细胞功能进行工程改造。在这里，我们描述了在模式生物酿酒酵母中两种化学诱导的二聚化系统。我们以自噬途径为例，展示了如何使用这些方法来剖析细胞中的分子事件。
