Maejo University, Chiang Mai 50290, Thailand.
Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, Canada.
Can J Microbiol. 2024 Dec 1;70(12):529-537. doi: 10.1139/cjm-2024-0071. Epub 2024 Aug 8.
Plant growth promotion by sp. 31-12 incorporated into a lettuce seed pelleting matrix was studied. We examined (1) the effect of five rhizosphere derived bacterial strains on green oak lettuce ( L.) seed germination, root and shoot growth, as a strain selection step for seed coating and seed pelletizing studies, (2) population stability of sp. 31-12 incorporated into a pelleting matrix on lettuce seed stored three months at 4 °C, and (3) lettuce growth promotion in the laboratory and greenhouse by sp. 31-12 coated and pelletized seed. A spontaneous streptomycin mutant of sp. 31-12 (str) was used to determine population size on seed and roots of 15- and 30-day-old lettuce. The population of sp. 31-12str on coated and pelleted seed decreased from 10 cfu/seed to 10 cfu/seed after 3 months storage at 4 °C. However, the population exceeded 10 cfu/g root dry mass and 10/g root dry mass after 15 days and 30 days in the greenhouse. Leaf fresh mass was significantly increased ( ≤ 0.05) with sp. 31-12 seed treatment as compared to noninoculated seed. In conclusion, pelletized lettuce seed with sp. 31-12 promoted growth and yield in the greenhouse.
研究了将 sp. 31-12 整合到生菜种子包衣基质中对植物生长的促进作用。我们考察了（1）五种根际衍生细菌菌株对绿橡生菜（L.）种子萌发、根和芽生长的影响，作为种子包衣和种子粒化研究的菌株选择步骤，（2）在 4°C 下储存三个月的包衣基质中 sp. 31-12 的种群稳定性，以及（3） sp. 31-12 包衣和粒化种子在实验室和温室中对生菜生长的促进作用。使用 sp. 31-12 的自发链霉素突变体（str）来确定 15 天和 30 天生菜种子和根上的种群大小。在 4°C 下储存 3 个月后， sp. 31-12str 在包衣和粒化种子上的种群数量从 10 cfu/种子减少到 10 cfu/种子。然而，在温室中 15 天和 30 天后，其种群数量超过了 10 cfu/g 根干重和 10 cfu/g 根干重。与未接种种子相比， sp. 31-12 种子处理显著增加了叶片鲜重（≤0.05）。总之，带有 sp. 31-12 的粒化生菜种子在温室中促进了生长和产量。
