Fang Jiaying, Yang Chuangye, Liao Yongshan, Wang Qingheng, Deng Yuewen
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Zhanjiang, 524088, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Dec;52:101304. doi: 10.1016/j.cbd.2024.101304. Epub 2024 Aug 2.
Pinctada fucata martensii is an economically important bivalve mollusk, as this species makes a major contribution to seawater pearl production. Pearl production efficiency varies between the sexes of P. f. martensii, but many aspects of the molecular mechanisms underlying sex determination and sex differentiation in P. f. martensii remain unclear. Here, transcriptomic and metabonomic analyses were conducted to identify the major genes and metabolic changes associated with sex determination and gametogenesis. We identified a total of 3426 differentially expressed genes (DEGs) between females and males. These included Fem-1c and Foxl2, which are involved in sex determination and sex differentiation, and SOHLH2, Nanos1 and TSSK4, which are involved in gametogenesis. We also identified a total of 5231 significant differential metabolites (SDMs) between females and males. These DEGs were enriched in 47 metabolic pathways, including "ABC transporters," "purine metabolism," and "glycerophospholipid metabolism." Our findings provide new insights into the molecular mechanisms underlying sex determination, sex differentiation, and gametogenesis and will aid future studies of P. f. martensii.
合浦珠母贝是一种具有重要经济价值的双壳贝类，因为该物种对海水珍珠生产做出了重大贡献。合浦珠母贝的珍珠生产效率在雌雄之间存在差异，但合浦珠母贝性别决定和性别分化的分子机制的许多方面仍不清楚。在此，进行了转录组学和代谢组学分析，以确定与性别决定和配子发生相关的主要基因和代谢变化。我们共鉴定出3426个雌雄之间的差异表达基因（DEG）。这些基因包括参与性别决定和性别分化的Fem-1c和Foxl2，以及参与配子发生的SOHLH2、Nanos1和TSSK4。我们还共鉴定出5231个雌雄之间的显著差异代谢物（SDM）。这些DEG富集在47条代谢途径中，包括“ABC转运蛋白”、“嘌呤代谢”和“甘油磷脂代谢”。我们的研究结果为性别决定、性别分化和配子发生的分子机制提供了新的见解，并将有助于未来对合浦珠母贝的研究。
