钴（II）-牛碳酸酐酶B-三氯乙醛体系的研究

Investigation of the system cobalt(II) bovine carbonic anhydrase B-trichloroacetaldehyde.

作者信息

Bertini I, Borghi E, Canti G, Luchinat C

出版信息

J Inorg Biochem. 1979 Aug;11(1):49-56. doi: 10.1016/s0162-0134(00)80053-7.

Abstract

The interactions between hydrated trichloroacetaldehyde and cobalt(II)bovine carbonic anhydrase B have been investigated as a function of pH by means of electronic spectroscopy of FT nmr spectroscopy. The hydrated aldehyde is bound to the metal ion and its apparent affinity constant is pH dependent with a bell-shaped profile. The kinetic parameters of the dissociation process have also been determined.

摘要

通过傅里叶变换核磁共振光谱的电子光谱法，研究了水合三氯乙醛与钴（II）牛碳酸酐酶B之间的相互作用随pH的变化情况。水合醛与金属离子结合，其表观亲和常数与pH有关，呈钟形曲线。还测定了解离过程的动力学参数。