Lei Ruiwen, Yao Chaoling, Huang Renfeng, Wu Wanming, Ou Linyang, Yang Chuansheng
Department of Head-Neck and Breast Surgery, Yuebei People's Hospital of Shantou University, Shaoguan, China.
Discov Oncol. 2024 Aug 9;15(1):339. doi: 10.1007/s12672-024-01196-6.
To investigate the effects of higher cellular stanniocalcin 2 (STC2) on suppressing the migration and invasion but promoting the apoptosis of triple-negative breast cancer (TNBC). STC2 in TNBC and the para-carcinoma tissues were analyzed by immunohistochemistry (IHC), while the mRNA level was measured by qPCR. Over-expressing or silencing STC2 was established in MDA-MB-231 cells. Epithelial mesenchymal transition (EMT) related proteins, cell migration, invasion, proliferation and apoptosis were detected. MDA-MB-231 with over-expressing or silencing STC2 were injected into nude mice to formatting tumors, and then EMT related proteins were measured by IHC. Lower STC2 expressed in TNBC tissues than in the para-carcinoma tissues. Silencing STC2 promoted EMT of TNBC cell MDA-MB-231, as well as cell migration, invasion and proliferation, but suppressed MDA-MB-231 apoptosis, while over-expressing STC2 had the opposite results, which might be related to PKC/PI3K/AKT/mTOR pathway. STC2 was the protective gene in TNBC, by suppressing migration and invasion to inhibit MDA-MB-231 cell EMT but promote cell apoptosis, in order to suppress TNBC progression.
研究高细胞水平的司坦钙素2（STC2）对抑制三阴性乳腺癌（TNBC）迁移和侵袭但促进其凋亡的影响。采用免疫组织化学（IHC）分析TNBC及癌旁组织中的STC2，同时用qPCR检测其mRNA水平。在MDA-MB-231细胞中建立STC2过表达或沉默模型。检测上皮间质转化（EMT）相关蛋白、细胞迁移、侵袭、增殖及凋亡情况。将过表达或沉默STC2的MDA-MB-231细胞注射到裸鼠体内形成肿瘤，然后用IHC检测EMT相关蛋白。TNBC组织中STC2的表达低于癌旁组织。沉默STC2可促进TNBC细胞MDA-MB-231的EMT，以及细胞迁移、侵袭和增殖，但抑制MDA-MB-231细胞凋亡，而过表达STC2则产生相反结果，这可能与PKC/PI3K/AKT/mTOR通路有关。STC2是TNBC中的保护基因，通过抑制迁移和侵袭来抑制MDA-MB-231细胞EMT但促进细胞凋亡，从而抑制TNBC进展。
