Qiao Yanjun, Gu Ming, Wang Xiaowei, Chen Rui, Kong Lingchao, Li Shuaitong, Li Jiaqi, Liu Qingkun, Hou Sibing, Wang Zeying
College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
Mol Biotechnol. 2024 Aug 8. doi: 10.1007/s12033-024-01244-0.
Exploring the landscape of protein phosphorylation, this investigation focuses on skin samples from LCG (Liaoning Cashmere Goats), characterized by different levels of cashmere fineness. Employing LC-MS/MS technology, we meticulously scrutinized FT-LCG (fine-type Liaoning Cashmere Goats) and CT-LCG (coarse-type Liaoning Cashmere Goats). Identifying 512 modified proteins, encompassing 1368 phosphorylated peptide segments and 1376 quantifiable phosphorylation sites, our exploration further revealed consistent phosphorylation sites in both groups. Analysis of phosphorylated peptides unveiled kinase substrates, prominently featuring Protein Kinase C, Protein Kinase B and MAPK3-MAPK1-MAPK7-NLK-group. Differential analysis spotlighted 28 disparate proteins, comprising six upregulated and twenty-two downregulated. Cluster analysis showcased the robust clustering efficacy of the two sample groups. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses underscored the significance of the purine metabolism pathway, suggesting its pivotal role in modulating cashmere fineness in LCG. Notably, through differential protein analysis, two crucial proteins were identified: HSL-X (hormone-sensitive lipase isoform X1) and KPRP (keratinocyte proline-rich protein). Further evidence supports LIPE and KPRP as key genes regulating cashmere fineness, paving the way for promising avenues in further research. These findings not only contribute to a nuanced understanding of protein-level dynamics in cashmere but also provide a theoretical foundation for the selective breeding of superior Liaoning Cashmere Goat strands.
通过探索蛋白质磷酸化的全貌，本研究聚焦于辽宁绒山羊（LCG）不同羊绒细度水平的皮肤样本。利用液相色谱-串联质谱（LC-MS/MS）技术，我们仔细研究了细型辽宁绒山羊（FT-LCG）和粗型辽宁绒山羊（CT-LCG）。我们鉴定出512种修饰蛋白，包括1368个磷酸化肽段和1376个可定量的磷酸化位点，进一步揭示了两组中一致的磷酸化位点。对磷酸化肽段的分析揭示了激酶底物，其中蛋白激酶C、蛋白激酶B以及MAPK3-MAPK1-MAPK7-NLK组尤为突出。差异分析突出了28种不同的蛋白质，其中6种上调，22种下调。聚类分析显示了两个样本组强大的聚类效果。基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析强调了嘌呤代谢途径的重要性，表明其在调节辽宁绒山羊羊绒细度方面的关键作用。值得注意的是，通过差异蛋白质分析，鉴定出了两种关键蛋白质：激素敏感脂肪酶异构体X1（HSL-X）和角质形成细胞富含脯氨酸蛋白（KPRP）。进一步的证据支持LIPE和KPRP作为调节羊绒细度的关键基因，为进一步研究开辟了有前景的途径。这些发现不仅有助于深入了解羊绒中蛋白质水平的动态变化，还为优质辽宁绒山羊品系的选育提供了理论基础。
