Astrada Adam, Nakagami Gojiro, Sanada Hiromi
School of Nursing, Faculty of Health Sciences, Esa Unggul University, Jakarta, Indonesia.
Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Int J Low Extrem Wounds. 2024 Aug 9:15347346241273112. doi: 10.1177/15347346241273112.
Foot ulcerations are one of the most common complications of diabetes and one of the major initial causes of amputations. The formation of biofilms on wounds significantly contributes to infections and delayed healing. While existing methods for identifying these biofilms have limitations, there is a need for a convenient tool for its clinical application. This literature review aimed to address the problem with current clinical biofilm identification in wound care and a proposal for biofilm-detection-based wound care in diabetic foot ulcer patients. Identifying biofilms is particularly vital due to the absence of typical signs of infection in DFUs. However, current approaches, although effective, often prove invasive and technically intricate. The wound blotting technique, involving attaching a nitrocellulose membrane and subsequent staining, presents an alternative that is swift and non-invasive. Research highlights the applicability of wound blotting with alcian blue staining in clinical scenarios, consistently producing sensitive outcomes. By addressing the critical need for early biofilm detection, wound blotting holds promise for enhancing DFU management and contributing to strategies aimed at preventing amputations.
足部溃疡是糖尿病最常见的并发症之一，也是截肢的主要初始原因之一。伤口上生物膜的形成显著导致感染和愈合延迟。虽然现有的识别这些生物膜的方法存在局限性，但仍需要一种便于临床应用的工具。这篇文献综述旨在解决伤口护理中当前临床生物膜识别存在的问题，并为糖尿病足溃疡患者基于生物膜检测的伤口护理提出建议。由于糖尿病足溃疡缺乏典型的感染迹象，识别生物膜尤为重要。然而，目前的方法虽然有效，但往往具有侵入性且技术复杂。伤口印迹技术，包括附着硝酸纤维素膜并随后染色，提供了一种快速且非侵入性的替代方法。研究强调了用阿尔辛蓝染色的伤口印迹在临床场景中的适用性，始终能产生敏感的结果。通过满足早期生物膜检测的迫切需求，伤口印迹有望改善糖尿病足溃疡的管理，并有助于制定旨在预防截肢的策略。
