Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090 Vienna, Austria.
Department of Dermatology, Medical University of Vienna, 1090 Vienna, Austria.
Cells. 2024 Aug 5;13(15):1308. doi: 10.3390/cells13151308.
Platelet-rich fibrin (PRF) is prepared by spontaneous coagulation of fractionated blood. When squeezed between two plates, PRF is separated into solid PRF membranes and a liquid exudate, the PRF serum. The question arises regarding how much the overall activity remains in the PRF membranes and what is discarded into the PRF serum. To this end, we have exposed gingival fibroblasts to lysates prepared from PRF membranes and PRF serum, followed by bulk RNA sequencing. A total of 268 up- and 136 down-regulated genes in gingival fibroblasts exposed to PRF membrane lysates were significantly regulated under the premise of a minimum log2 with 2.5-fold change and a minus log10 significance level of two, respectively. PRF serum only caused 62 up- and 32 down-regulated genes under these conditions. Among the 46 commonly up-regulated genes were CXCL1, CXCL5, CXCL6, CXCL8, IL33, IL6, and PTGS2/COX2, stanniocalcin-1-all linked to an inflammatory response. PRF membrane lysates further increased chemokines CCL2, CCL7, CXCL2, CXCL3, and IL1R1, IL1RL1, and IL1RN, as well as the paracrine factors IL11, LIF, IGF1, BMP2, BMP6, FGF2, and CCN2/CTGF, and all hyaluronan synthases. On the other hand, PRF serum increased DKK1. The genes commonly down-regulated by PRF membrane lysates and PRF serum included interferon-induced protein with tetratricopeptide repeats (IFIT1, IFIT2, IFIT3) and odd-skipped-related transcription factors (OSR1 and OSR2), as well as FGF18 and GDF15, respectively. Taken together, PRF membrane lysates, compared to PRF serum, cause a more complex response in gingival fibroblasts, but each increased chemokine expression in gingival fibroblasts.
富血小板纤维蛋白 (PRF) 通过分离血液的自然凝固来制备。当在两个板之间挤压时，PRF 被分离成固体 PRF 膜和液体渗出物，即 PRF 血清。问题是 PRF 膜中保留了多少整体活性，以及哪些被丢弃到 PRF 血清中。为此，我们已经使牙龈成纤维细胞暴露于 PRF 膜和 PRF 血清的裂解物中，然后进行批量 RNA 测序。在前提是最小对数 2.5 倍变化和负对数 10 显著性水平为 2 的情况下，暴露于 PRF 膜裂解物的牙龈成纤维细胞中，有 268 个上调基因和 136 个下调基因显著调节。在这些条件下，PRF 血清仅引起 62 个上调基因和 32 个下调基因。在 46 个共同上调基因中，包括 CXCL1、CXCL5、CXCL6、CXCL8、IL33、IL6 和 PTGS2/COX2、STC1-1 全都与炎症反应有关。PRF 膜裂解物进一步增加趋化因子 CCL2、CCL7、CXCL2、CXCL3 和 IL1R1、IL1RL1 和 IL1RN，以及旁分泌因子 IL11、LIF、IGF1、BMP2、BMP6、FGF2 和 CCN2/CTGF 和所有透明质酸合酶。另一方面，PRF 血清增加了 DKK1。PRF 膜裂解物和 PRF 血清共同下调的基因包括干扰素诱导的四肽重复蛋白 (IFIT1、IFIT2、IFIT3) 和奇数跳过相关转录因子 (OSR1 和 OSR2)，以及 FGF18 和 GDF15。总之，与 PRF 血清相比，PRF 膜裂解物在牙龈成纤维细胞中引起更复杂的反应，但每种物质都增加了牙龈成纤维细胞中的趋化因子表达。
