O'Shannessy D J, Quarles R H
J Appl Biochem. 1985 Aug-Oct;7(4-5):347-55.
Methods for the specific conjugation of both polyclonal antibodies and monoclonal antibodies via their carbohydrate moieties are described. Mild oxidation of the immunoglobulin with sodium periodate produces reactive aldehydes on the carbohydrate moieties. Subsequent reaction with hydrazide derivatives of biotin, fluorescent dyes, or enzymes produces stable antibody conjugates which retain full immunological activity. In addition, immunoaffinity supports can be prepared in the same manner using solid supports containing a hydrazide function.
本文描述了通过多克隆抗体和单克隆抗体的碳水化合物部分进行特异性偶联的方法。用高碘酸钠对免疫球蛋白进行温和氧化，可在碳水化合物部分产生反应性醛基。随后与生物素、荧光染料或酶的酰肼衍生物反应，可产生保留完全免疫活性的稳定抗体偶联物。此外，使用含有酰肼功能的固体支持物，可通过相同方式制备免疫亲和支持物。
