Xu Yi, Zhang Yanshu, Ma Funing, Zhao Jingxi, Yang Huiting, Song Shun, Zhang Shaoling
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Sanya Institute of Nanjing Agricultural University, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory of Biological Breeding for Tropical Crops, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Plants (Basel). 2024 Jul 31;13(15):2119. doi: 10.3390/plants13152119.
Bananas are one of the most important cash crops in the tropics and subtropics. Drought and low-temperature stress affect the growth of banana. The (dehydration responsive element binding protein) gene family, as one of the major transcription factor families, plays crucial roles in defense against abiotic stress. Currently, systematic analyses of the banana () gene family have not yet been reported. In this study, 103 members of the gene family were identified in the banana genome. In addition, transcriptomic analysis results revealed that responded to drought and cold stress. The expression of was induced by drought and cold stress; these geneswere selected for further analysis. The qRT-PCR validation results confirmed the transcriptome results. Additionally, transgenic Arabidopsis plants overexpressing exhibited enhanced resistance to drought and cold stress by reducing MDA content and increasing PRO and soluble sugar content. This study enhances our understanding of the function of the gene family, provides new insights into their regulatory role under abiotic stress, and lays a good foundation for improving drought and cold stress-tolerant banana verities.
香蕉是热带和亚热带地区最重要的经济作物之一。干旱和低温胁迫会影响香蕉的生长。脱水响应元件结合蛋白(dehydration responsive element binding protein)基因家族作为主要的转录因子家族之一,在抵御非生物胁迫中发挥着关键作用。目前,尚未见对香蕉脱水响应元件结合蛋白(dehydration responsive element binding protein)基因家族的系统分析报道。在本研究中,在香蕉基因组中鉴定出了103个脱水响应元件结合蛋白(dehydration responsive element binding protein)基因家族成员。此外,转录组分析结果显示,脱水响应元件结合蛋白(dehydration responsive element binding protein)对干旱和寒冷胁迫有响应。脱水响应元件结合蛋白(dehydration responsive element binding protein)的表达受干旱和寒冷胁迫诱导;选择这些基因进行进一步分析。qRT-PCR验证结果证实了转录组结果。此外,过表达脱水响应元件结合蛋白(dehydration responsive element binding protein)的转基因拟南芥植株通过降低丙二醛(MDA)含量、增加脯氨酸(PRO)和可溶性糖含量,表现出对干旱和寒冷胁迫的抗性增强。本研究增进了我们对脱水响应元件结合蛋白(dehydration responsive element binding protein)基因家族功能的理解,为其在非生物胁迫下的调控作用提供了新见解,并为培育耐旱耐寒香蕉品种奠定了良好基础。