Research Center of Neurology, 80 Volokolamskoe Shosse, 125367 Moscow, Russia.
Int J Mol Sci. 2024 Jul 25;25(15):8113. doi: 10.3390/ijms25158113.
Age-dependent cerebral small vessel disease (CSVD) is a common disease with a high social burden characterized by heterogeneity of forms and frequent comorbidity with Alzheimer's disease (AD). Previously, we identified two MRI types of CSVD with specific clinical presentation and, probably, different mechanisms. The present study included 34 patients with CSVD and white matter hyperintensity (WMH) of stage Fazekas (F) 3 (mean age 61.7 ± 8.9) and 11 volunteers (mean age 57.3 ± 9.7). Total RNA was isolated from peripheral blood leukocytes. The expression of 58 protein-coding genes associated with CSVD and/or AD and 4 reference genes were assessed as part of the original panel for the NanoString nCounter analyzer. Testing results were validated by real-time PCR. There was a significant decrease in the expression levels of the , , , , and genes in MRI type 2 relative to the control group as well as a decrease in the expression level of the gene in MRI type 2 compared to MRI type 1. Processes associated with inflammatory pathways with decreased expression of the identified genes are important in the development of MRI type 2 of CSVD. Given the direct connection of the established genes with AD, the importance of this form of CSVD in comorbidity with AD has been assumed.
年龄相关性脑小血管病（CSVD）是一种具有高度社会负担的常见疾病，其特征为形式的异质性和与阿尔茨海默病（AD）的频繁合并症。以前，我们确定了两种具有特定临床表现且可能具有不同机制的 MRI 类型的 CSVD。本研究包括 34 例 CSVD 伴脑白质高信号（WMH）Fazekas 分期 3 级（平均年龄 61.7 ± 8.9）的患者和 11 名志愿者（平均年龄 57.3 ± 9.7）。从外周血白细胞中分离总 RNA。使用 NanoString nCounter 分析器的原始试剂盒评估了与 CSVD 和/或 AD 相关的 58 个蛋白编码基因和 4 个参考基因的表达情况。通过实时 PCR 验证了检测结果。与对照组相比，MRI 类型 2 中 、 、 、 和 基因的表达水平显著降低，与 MRI 类型 1 相比，MRI 类型 2 中 基因的表达水平也降低。与炎症途径相关的过程中，鉴定出的基因表达降低，这在 MRI 类型 2 的 CSVD 发展中很重要。鉴于已建立的基因与 AD 直接相关，因此假定这种形式的 CSVD 与 AD 的合并症有关。
