Richards S J, Morris R J, Raisman G
Neuroscience. 1985 Nov;16(3):617-23. doi: 10.1016/0306-4522(85)90196-4.
A small but distinctive population (about 1 in 600) of magnocellular neurosecretory neurons in homozygous Brattleboro rats are immunoreactive for vasopressin, and a similar number for the carboxy-terminal glycopeptide of the vasopressin prohormone. These solitary cells are found in all animals and in all parts of the magnocellular system, but not in the suprachiasmatic or other hypothalamic nuclei. The majority of the solitary cells do not differ morphologically from the remainder of the magnocellular neurons. The immunoreactivity is markedly denser in the Nissl bodies than in the Golgi region. Serial sections show that the vasopressin and glycopeptide immunoreactive material is co-localized in the same cells, and that these cells are not immunoreactive for oxytocin. A published sequence for the Brattleboro vasopression gene mutation indicates a base-deletion upstream from the glycopeptide-encoding portion, and implies a frameshift that would cause translation of incorrect protein continuing into the poly-A tail of the mRNA. Although this could apply to the majority of the Brattleboro presumptive vasopressin neurons, the co-localization in our solitary cells of material immunoreactive with antibodies to both the amino- and carboxy-terminals of the vasopressin prohormone suggest that in these cases an additional mechanism may be operating.
纯合布拉特洛伯大鼠中一小部分但具有显著特征的大细胞神经分泌神经元群体（约每600个中有1个）对加压素具有免疫反应性，对加压素前激素的羧基末端糖肽具有免疫反应性的细胞数量与之相似。这些单个细胞在所有动物的大细胞系统的所有部位均有发现，但在视交叉上核或其他下丘脑核中未发现。大多数单个细胞在形态上与大细胞神经元的其余部分并无差异。免疫反应性在尼氏体中明显比在高尔基体区域更密集。连续切片显示，加压素和糖肽免疫反应性物质共定位于同一细胞中，并且这些细胞对催产素无免疫反应性。已发表的布拉特洛伯加压素基因突变序列表明，在糖肽编码部分上游存在碱基缺失，并暗示会导致移码，从而使不正确的蛋白质翻译持续到mRNA的聚腺苷酸尾。尽管这可能适用于大多数布拉特洛伯假定的加压素神经元，但我们的单个细胞中与加压素前激素的氨基末端和羧基末端抗体均具有免疫反应性的物质的共定位表明，在这些情况下可能存在另一种机制在起作用。
