Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Perth, Australia.
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115201, Taiwan.
BMC Microbiol. 2024 Aug 10;24(1):299. doi: 10.1186/s12866-024-03454-7.
The fungus Parastagonospora nodorum causes septoria nodorum blotch on wheat. The role of the fungal Velvet-family transcription factor VeA in P. nodorum development and virulence was investigated here. Deletion of the P. nodorum VeA ortholog, PnVeA, resulted in growth abnormalities including pigmentation, abolished asexual sporulation and highly reduced virulence on wheat. Comparative RNA-Seq and RT-PCR analyses revealed that the deletion of PnVeA also decoupled the expression of major necrotrophic effector genes. In addition, the deletion of PnVeA resulted in an up-regulation of four predicted secondary metabolite (SM) gene clusters. Using liquid-chromatography mass-spectrometry, it was observed that one of the SM gene clusters led to an accumulation of the mycotoxin alternariol. PnVeA is essential for asexual sporulation, full virulence, secondary metabolism and necrotrophic effector regulation.
真菌禾旋孢腔菌（Parastagonospora nodorum）可引起小麦叶枯病。本研究旨在探究真菌 Velvet 家族转录因子 VeA 在禾旋孢腔菌发育和毒性中的作用。禾旋孢腔菌 VeA 直系同源物 PnVeA 的缺失导致生长异常，包括色素沉着、无性孢子形成能力丧失以及对小麦的致病性显著降低。比较 RNA-Seq 和 RT-PCR 分析表明，PnVeA 的缺失还使主要坏死性效应因子的表达脱耦联。此外，PnVeA 的缺失导致四个预测的次生代谢（SM）基因簇的上调。使用液相色谱-质谱法观察到，一个 SM 基因簇导致真菌毒素交链孢酚的积累。PnVeA 对于无性孢子形成、完全毒性、次生代谢和坏死性效应因子调控是必需的。
