Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao, Shandong Province, China.
Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2024 Nov;138(5):626-634. doi: 10.1016/j.oooo.2024.07.005. Epub 2024 Jul 22.
U2AF homology motif kinase 1 (UHMK1) is a newly discovered molecule that may have multiple functions. Recent studies have revealed that UHMK1 had aberrant expression in many tumors and was associated with tumor progression. However, UHMK1 was rarely reported in oral squamous cell carcinoma (OSCC).
In this study, Western blot, quantitative real-time polymerase chain reaction (PCR), and immunohistochemistry were used to detect the expression of UHMK1 in OSCC and peritumoral non-neoplastic tissues. Then, its relationship with clinicopathologic parameters was analyzed. The Kaplan-Meier method and Cox regression model were used to analyze the effects of UHMK1 expression on the prognosis and survival of OSCC patients.
Our results showed that UHMK1 had higher expression in OSCC tissues compared with in peritumoral non-neoplastic tissues, and its high expression was associated with high TNM stage and lymph node metastasis. High UHMK1 expression was related to short overall and disease-free survival times. Moreover, UHMK1 expression was identified as an independent prognostic factor that influences overall and disease-free survival of OSCC patients.
High expression of UHMK1 is associated with the poor prognosis of patients, and it can be used as a potential prognostic molecule for OSCC.
U2AF 同源结构域激酶 1（UHMK1）是一种新发现的分子，可能具有多种功能。最近的研究表明，UHMK1 在许多肿瘤中存在异常表达，并与肿瘤进展有关。然而，UHMK1 在口腔鳞状细胞癌（OSCC）中很少被报道。
本研究采用 Western blot、定量实时聚合酶链反应（PCR）和免疫组织化学方法检测 UHMK1 在 OSCC 及癌旁非肿瘤组织中的表达情况，并分析其与临床病理参数的关系。采用 Kaplan-Meier 法和 Cox 回归模型分析 UHMK1 表达对 OSCC 患者预后和生存的影响。
我们的结果表明，UHMK1 在 OSCC 组织中的表达高于癌旁非肿瘤组织，其高表达与 TNM 分期高和淋巴结转移有关。高 UHMK1 表达与总生存期和无病生存期较短有关。此外，UHMK1 表达被确定为影响 OSCC 患者总生存期和无病生存期的独立预后因素。
UHMK1 的高表达与患者的不良预后相关，可作为 OSCC 的潜在预后分子。
