Sánchez-Tito Marco, Tay Lidia-Yileng, Zea-Gamboa Francisco, Cartagena-Cutipa Raúl, Flores-Gómez Alysson, Spigno-Paco Bruno, Cadenas Tania-Coral-Hernandez, Díaz Ingrit-Elida-Collantes
Faculty of Health Sciences, Universidad Privada de Tacna, Tacna, Peru.
Faculty of Stomatology, Universidad Peruana Cayetano Heredia, Lima, Peru.
J Clin Exp Dent. 2024 Jun 1;16(6):e670-e677. doi: 10.4317/jced.61356. eCollection 2024 Jun.
To evaluate the effectiveness of an experimental toothpaste based on Juss. essential oil against a bacterial consortium associated with dental caries.
The antibacterial activity of the essential oil was evaluated by the disk diffusion and microdilution tests against , and . Petri dishes were incubated at 37°C for 24 h. An experimental toothpaste was prepared at a concentration of 201.76 mg/mL. The antibacterial activity of the experimental and six commercial toothpastes were evaluated by agar well-diffusion method. Petri dishes were inoculated with a bacterial consortium prepared with the three strains. 80 mg of the toothpastes were placed in the wells and the Petri dishes were incubated at 37°C for 24 h. The inhibition zones were measured with a digital compass. The differences between the pastas were evaluated with the one-way ANOVA test, with a 5% level of significance.
The essential oil was more effective than 0.12% chlorhexidine in inhibiting the growth of (29.02±1.74 mm) and (21.92±3.43 mm), being more moderate for (17.66±1.11 mm) . In MBC tests, the EO showed complete inhibition of the growth of at a concentration of 5% (50.44 mg/mL), 10% (100.88 mg/mL) for and 2.5% (25.22 mg/mL) for . The experimental toothpaste was effective in inhibiting the growth of the bacterial consortium (31.88±66 mm), having a similar performance to Total Dent, Colgate Total 12, Kolynos® Herbal and Colgate® Herbal (> 0.05).
The development of an experimental paste based on H. laricifolium Juss. essential oil (0.28% v/v) showed an important antibacterial activity similar to commercial toothpastes against a bacterial consortium of , and . Antibacterial agents, toothpastes, medicinal plants, Streptococcus mutans, Streptococcus sanguinis, Streptococcus salivarius.
评估一种基于柳叶菜精油的实验性牙膏对与龋齿相关的细菌群落的有效性。
通过纸片扩散法和微量稀释法评估该精油对变形链球菌、血链球菌和唾液链球菌的抗菌活性。培养皿在37°C下孵育24小时。制备浓度为201.76 mg/mL的实验性牙膏。通过琼脂孔扩散法评估实验性牙膏和六种市售牙膏的抗菌活性。用这三种菌株制备细菌群落接种于培养皿。将80 mg牙膏置于孔中，培养皿在37°C下孵育24小时。用数字罗盘测量抑菌圈。用单因素方差分析检验评估牙膏之间的差异，显著性水平为5%。
该精油在抑制变形链球菌（29.02±1.74 mm）和血链球菌（21.92±3.43 mm）生长方面比0.12%洗必泰更有效，对唾液链球菌（17.66±1.11 mm）的抑制作用较弱。在最低杀菌浓度试验中，该精油在浓度为5%（50.44 mg/mL）时对变形链球菌的生长有完全抑制作用，对血链球菌为10%（100.88 mg/mL），对唾液链球菌为2.5%（25.22 mg/mL）。实验性牙膏对细菌群落的生长有抑制作用（31.88±66 mm），其性能与全效牙膏、高露洁全效12、科利诺斯草本牙膏和高露洁草本牙膏相似（>0.05）。
基于柳叶菜精油（0.28% v/v）开发的实验性牙膏对变形链球菌、血链球菌和唾液链球菌的细菌群落显示出与市售牙膏相似的重要抗菌活性。抗菌剂、牙膏、药用植物、变形链球菌、血链球菌、唾液链球菌
