Tanweer Sana, Sharma Tarina, Grover Abhinav, Agarwal Meetu, Grover Sonam
Department of Molecular Medicine, Jamia Hamdard, New Delhi-110065, India.
New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey 07103, United States.
ACS Omega. 2024 Jul 23;9(31):33743-33750. doi: 10.1021/acsomega.4c02919. eCollection 2024 Aug 6.
A essential gene, ThyX (Rv2754c), plays a key role in intermediate metabolism and respiration by catalyzing the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate. ThyX is present in the complex and in a nonpathogenic strain of Mycobacteria. In this study, we identified a novel function of ThyX, an enzyme with immune-modulating properties. We have shown that ThyX can activate the macrophages in the host toward M1 response. Overexpression of ThyX stimulates the production of nitrite oxide (NO) and induces apoptosis in macrophages; indeed both responses help the host to control growth of . ThyX was also discovered to play a role in the recombinant bacterium's ability to survive when it was subjected to oxidative and hypoxic stress by macrophages. These findings demonstrate the protein's functional importance in . Indeed these findings represent ThyX as a potential candidate for future research and show this as a therapeutic target.
一个必需基因，ThyX（Rv2754c），通过催化由dUMP和亚甲基四氢叶酸形成dTMP和四氢叶酸，在中间代谢和呼吸中起关键作用。ThyX存在于结核分枝杆菌的复合体和非致病菌株中。在本研究中，我们鉴定了ThyX的一种新功能，它是一种具有免疫调节特性的酶。我们已经表明，ThyX可以激活宿主中的巨噬细胞向M1反应。ThyX的过表达刺激一氧化氮（NO）的产生并诱导巨噬细胞凋亡；事实上，这两种反应都有助于宿主控制结核分枝杆菌的生长。还发现ThyX在重组细菌受到巨噬细胞的氧化和缺氧应激时的存活能力中起作用。这些发现证明了该蛋白在结核分枝杆菌中的功能重要性。确实，这些发现表明ThyX是未来研究的潜在候选者，并显示其为治疗靶点。
