Xiong Haibing, Jiang Ran, Xing Lingzhi, Zheng Jiaojiao, Tian Xinhong, Leng Jiajie, Guo Xin, Zeng Shi, Xiong Haofeng, Huo Jianhong, Li Letai
Banan Hospital Affiliated to Chongqing Medical University, Chongqing, China.
The First College of Clinical Medicine, Chongqing Medical University, Chongqing, China.
Front Neurol. 2024 Jul 26;15:1423569. doi: 10.3389/fneur.2024.1423569. eCollection 2024.
Previous observational clinical studies and meta-analyses have yielded inconsistent results regarding the relationship between vitamin D and headache, and the causal relationship remains unclear. The aim of this study was to investigate the causal relationship between vitamin D and headache by bidirectional two-sample Mendelian randomisation (MR) analysis.
The relationship between high levels of vitamin D and headache was investigated by two-sample MR analysis using publicly available genome-wide association study (GWAS) data. The primary method was inverse variance weighting (IVW), and secondary methods were weighted median and MR-Egger methods. No heterogeneity or horizontal multidirectionality was found in the MR results. The robustness and validity of the findings were assessed using the leave-behind method.
A significant causal relationship was found between high vitamin D levels and headache using the IVW method (OR = 0.848; = 0.007; 95% CI = 0.752-0.956). However, in a reverse analysis, no evidence of a causal relationship between headache and high levels of vitamin D was found using the IVW method (OR = 1.001; = 0.906; 95% CI = 0.994-1.006). Our MR analyses showed no significant horizontal multidimensionality or heterogeneity ( > 0.05). Sensitivity analyses confirmed that MR estimates were not affected by single nucleotide polymorphisms (SNPs). Confirmation that our results are robust and valid has been obtained by the leave-one-out method.
Our study suggests that high levels of vitamin D prevent the risk of headache. However, there is no evidence of a causal relationship between headache and high levels of vitamin D. Vitamin D may reduce the risk of headache.
先前的观察性临床研究和荟萃分析在维生素D与头痛之间的关系上得出了不一致的结果，因果关系仍不明确。本研究的目的是通过双向双样本孟德尔随机化（MR）分析来研究维生素D与头痛之间的因果关系。
使用公开可用的全基因组关联研究（GWAS）数据，通过双样本MR分析研究高维生素D水平与头痛之间的关系。主要方法是逆方差加权（IVW），次要方法是加权中位数和MR-Egger方法。在MR结果中未发现异质性或水平多向性。使用留一法评估研究结果的稳健性和有效性。
使用IVW方法发现高维生素D水平与头痛之间存在显著的因果关系（OR = 0.848；P = 0.007；95%CI = 0.752 - 0.956）。然而，在反向分析中，使用IVW方法未发现头痛与高维生素D水平之间存在因果关系的证据（OR = 1.001；P = 0.906；95%CI = 0.994 - 1.006）。我们的MR分析未显示出显著的水平多维度性或异质性（P>0.05）。敏感性分析证实MR估计不受单核苷酸多态性（SNP）的影响。留一法已证实我们的结果是稳健和有效的。
我们的研究表明，高维生素D水平可预防头痛风险。然而，没有证据表明头痛与高维生素D水平之间存在因果关系。维生素D可能会降低头痛风险。
