Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN, United States.
Department of Medicine, University of Minnesota, Minneapolis, MN, United States.
Front Immunol. 2024 Jul 26;15:1418539. doi: 10.3389/fimmu.2024.1418539. eCollection 2024.
CD177 plays an important role in the proliferation and differentiation of myeloid lineage cells including neutrophils, myelocytes, promyelocytes, megakaryocytes, and early erythroblasts in bone marrow. CD177 deficiency is a common phenotype in humans. Our previous studies revealed genetic mechanisms of human CD177 deficiency and expression variations. Up to now, immune functions of CD177 remain undefined. In the current study, we revealed human IgG as a ligand for CD177 by using flow cytometry, bead-rosette formation, and surface plasmon resonance (SPR) assays. In addition, we show that CD177 variants affect the binding capacity of CD177 for human IgG. Furthermore, we show that the CD177 genetic variants significantly affect antibody-dependent cell-mediated cytotoxicity (ADCC) function. The demonstration of CD177 as a functional IgG Fc-receptor may provide new insights into CD177 immune function and genetic mechanism underlying CD177 as biomarkers for human diseases.
CD177 在骨髓中髓系细胞（包括中性粒细胞、髓系细胞、早幼粒细胞、巨核细胞和早期红细胞）的增殖和分化中发挥重要作用。CD177 缺乏是人类的一种常见表型。我们之前的研究揭示了人类 CD177 缺乏和表达变异的遗传机制。到目前为止，CD177 的免疫功能仍未确定。在本研究中，我们通过流式细胞术、珠玫瑰花形成和表面等离子体共振（SPR）测定发现人 IgG 是 CD177 的配体。此外，我们表明 CD177 变体影响 CD177 与人 IgG 的结合能力。此外，我们表明 CD177 遗传变异显著影响抗体依赖性细胞介导的细胞毒性（ADCC）功能。CD177 作为功能性 IgG Fc 受体的证明可能为 CD177 的免疫功能和作为人类疾病生物标志物的 CD177 遗传机制提供新的见解。
