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Radioiodination of a photoactivatable heterobifunctional reagent.

作者信息

Ji I, Shin J, Ji T H

出版信息

Anal Biochem. 1985 Dec;151(2):348-9. doi: 10.1016/0003-2697(85)90186-1.

DOI:10.1016/0003-2697(85)90186-1
PMID:3913331
Abstract

The N-hydroxysuccinimide ester of 4-azidosalicylic acid, a photoactivable heterobifunctional reagent, can be radioiodinated. The low efficiency (3%) of the radioiodination by a previously published method (I. Ji and T. H. Ji, 1982, Anal. Biochem. 121, 286-289) has been increased to 63% by substituting the solvent, acetone, with others such as aqueous acetonitrile, dimethylformamide, or dimethyl sulfoxide. The resulting 125I reagent was used for derivatizing human choriogonadotropin. The radioactive hormone derivative was crosslinked to the alpha beta dimer upon photolysis.

摘要

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