Vogeli G, Horn E, Laurent M, Nath P
Anal Biochem. 1985 Dec;151(2):442-4. doi: 10.1016/0003-2697(85)90202-7.
A typical cDNA library with a large number of initial transformants, plated in soft agarose, can be stored and shipped in 12% glycerol at -70 degrees C. To prepare the library for storage, the soft agarose layer is made into a paste and the agarose is removed by Sephadex G-25 filtration. This method of cDNA library storage does not alter the relative representation of the plasmids carried in the library. To achieve a very uniform distribution of colonies at high colony density, an aliquot of the cDNA library is diluted to 3000 to 10,000 colonies/ml. One milliliter of this suspension is evenly distributed on a nitrocellulose filter on an agar plate and air-dried. Filter copies are made and screened by published methods.
一个含有大量初始转化子的典型cDNA文库，铺在软琼脂糖中，可以在-70℃下用12%甘油储存和运输。为了准备储存文库，将软琼脂糖层制成糊状物，并通过葡聚糖G-25过滤去除琼脂糖。这种cDNA文库储存方法不会改变文库中携带的质粒的相对代表性。为了在高菌落密度下实现菌落的非常均匀分布，将cDNA文库的一份等分试样稀释至3000至10000个菌落/毫升。将1毫升这种悬浮液均匀分布在琼脂平板上的硝酸纤维素滤膜上并风干。制作滤膜复制品并通过已发表的方法进行筛选。
