Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, 435002, Hubei province, China.
Hubei Provincial Key Laboratory for Quality and Safety of Traditional Chinese Medicine, Jing Brand Co., Ltd, Huangshi, 435002, Hubei province, China.
Plant Foods Hum Nutr. 2024 Dec;79(4):795-802. doi: 10.1007/s11130-024-01217-3. Epub 2024 Aug 12.
The Houttuynia cordata Thunb. belongs to the Saururaceae family and is a well-known medicine and food homologous plant. Herein, the isolation of an α-glucosidase inhibitor from Houttuynia cordata Thunb. and characterization of its in vitro and in vivo hypoglycemic bioactivities are reported. We optimized the extraction conditions and isolated neochlorogenic acid (nCGA), which has α-glucosidase inhibitory activity from Houttuynia cordata Thunb. for the first time. nCGA competed with glucose for the α-glucosidase binding site, with a 50% inhibitory concentration (IC) of 0.711 mg/mL. In vivo experiments in zebrafish showed that effects of nCGA on blood glucose varied by its concentrations. In particular, 4 mg/L nCGA significantly decreased the blood glucose level and inhibited effects of α-glucosidase in zebrafish. This work provides a theoretical basis for the extraction of hypoglycemic active ingredients from Houttuynia cordata Thunb. and a foundation for the development of natural and effective α-glucosidase inhibitors.
鱼腥草属于三白草科，是一种著名的药食同源植物。本研究报道了鱼腥草中α-葡萄糖苷酶抑制剂的分离及其体外和体内降血糖生物活性。我们优化了鱼腥草的提取条件，首次分离得到具有α-葡萄糖苷酶抑制活性的新绿原酸（nCGA）。nCGA 与葡萄糖竞争 α-葡萄糖苷酶结合位点，其 50%抑制浓度（IC）为 0.711mg/mL。在斑马鱼体内实验中，nCGA 对血糖的影响因浓度而异。特别是 4mg/L 的 nCGA 能显著降低斑马鱼的血糖水平并抑制α-葡萄糖苷酶的活性。这项工作为鱼腥草中降血糖活性成分的提取提供了理论依据，为开发天然、有效的α-葡萄糖苷酶抑制剂奠定了基础。
