Yao Shenggen, Meikle Thomas G, Keizer David W, Separovic Frances
Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC 3010, Australia.
Baker Heart and Diabetes Institute, Melbourne, VIC 3004, Australia.
J Phys Chem Lett. 2024 Aug 22;15(33):8520-8525. doi: 10.1021/acs.jpclett.4c01894. Epub 2024 Aug 12.
In model membrane systems, such as lipidic cubic phases (LCPs), H NMR spectra are dominated by resonances from water and lipid molecules. The measurement of translational diffusion of peptides/molecules encapsulated in LCPs using conventional H pulsed-gradient spin-echo (PGSE) NMR is, therefore, immensely difficult due to the dynamic range caused by the intense resonance(s) from the surrounding environment. The present study reports the use of a band-selective short transient PGSE sequence, avoiding the perturbation of both hydration water and lipids, for measuring the diffusion of molecules encapsulated within the lipid bilayer and the aqueous channels of LCPs.
在模型膜系统中，如脂质立方相（LCPs），氢核磁共振（H NMR）光谱主要由水和脂质分子的共振信号主导。因此，由于周围环境强烈共振所导致的动态范围，使用传统的氢脉冲梯度自旋回波（PGSE）核磁共振技术来测量包裹在LCPs中的肽/分子的平移扩散极其困难。本研究报道了使用一种带选择性的短瞬态PGSE序列，该序列可避免对水化水和脂质的干扰，用于测量包裹在脂质双层和LCPs水通道内分子的扩散。
