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从羽扇豆中提取水溶性多糖及其在酸性条件下对蛋白质的分散和稳定作用。

Extraction of water-soluble polysaccharides from lupin beans and their function of protein dispersion and stabilization under acidic conditions.

机构信息

Ibaraki University, College of Agriculture, Chuo 3-21-1, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan.

Ibaraki University, College of Agriculture, Chuo 3-21-1, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan; Fuji Oil Holdings Inc., Tsukuba R&D Center, 4-3 Kinunodai, Tsukubamirai, Ibaraki 300-2497, Japan.

出版信息

Int J Biol Macromol. 2024 Oct;278(Pt 3):134664. doi: 10.1016/j.ijbiomac.2024.134664. Epub 2024 Aug 10.

Abstract

In the current environment whereby new sources of proteins are extracted from plant material, it is also important to study the potential use of the resulting side streams. Although a number of studies have been conducted on various polysaccharides extracted from plant raw material, a polysaccharide fraction extracted from lupin bean is yet to be explored, in spite of the emerging interest in this crop as a source of food ingredients. In this work lupin soluble polysaccharide (LuPS) was obtained with a recovery as high as 46 % by extraction at pH 8, 120 °C, for 90 min. This fraction, named LuPS-8, was composed of a mostly linear pectic polysaccharide with a weight average molecular mass of 6608 kg/mol, and containing 71.0 % galactose, with minor amounts of arabinose (16.0 %), glucuronic acid 4.6 %, and galacturonic acid 4.1 %. When added to an acid milk dispersion, LuPS-8 improved its dispersibility, providing storage stability against sedimentation over a wider pH range than a HM-pectin reference, between 3.6-4.4. This research demonstrated the potential for upcycling of a side stream of lupin protein production, by the creation of value-added novel functional polysaccharide.

摘要

在当前从植物材料中提取新蛋白质来源的环境下,研究由此产生的副产物的潜在用途也很重要。尽管已经对从植物原料中提取的各种多糖进行了许多研究,但从羽扇豆中提取的多糖部分尚未得到探索,尽管人们对这种作物作为食品成分的来源越来越感兴趣。在这项工作中,通过在 pH 值为 8、120°C 下提取 90 分钟,从羽扇豆中获得了回收率高达 46%的可溶性羽扇豆多糖(LuPS)。该级分命名为 LuPS-8,由主要线性果胶多糖组成,重均分子量为 6608kg/mol,含有 71.0%的半乳糖,少量阿拉伯糖(16.0%)、4.6%的葡萄糖醛酸和 4.1%的半乳糖醛酸。当添加到酸性乳分散体中时,LuPS-8 提高了其分散性,在更宽的 pH 范围内提供了比 HM-果胶参考物更好的储存稳定性,防止沉淀,pH 值范围为 3.6-4.4。这项研究表明,通过创造有价值的新型功能性多糖,可以对羽扇豆蛋白生产的副产物进行再利用。

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