Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Ooty, 643001, The Nilgiris, Tamilnadu, India.
Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Ooty, 643001, The Nilgiris, Tamilnadu, India.
J Ethnopharmacol. 2024 Dec 5;335:118691. doi: 10.1016/j.jep.2024.118691. Epub 2024 Aug 10.
'Karkataka Taila (KT), an ancient Ayurvedic Rasayana comprising the edible freshwater crab Scylla serrata Forskal flesh, is still used by local traditional practitioners in Kerala state to treat tremors and palsy. In the scientific community, it becomes less exposed due to the lack of adequate scientific validations and brief reports. There has been no published research on the effectiveness of KT in treating Parkinson's disease (PD).
The purpose of the current research work was to investigate the anti-Parkison's potential of KT against rotenone-induced neurotoxicity in SH-SY5Y cell lines and rat model of PD and investigate underlying molecular mechanisms.
The components of KT have been identified by gas chromatography-mass spectroscopy (GC-MS). The neuroprotective activity of KT was assessed using SH-SY5Y cell lines and rats against rotenone-induced PD. The parameters used for asses the neuroprotection are antioxidant markers (ROS and SOD), anti-inflammatory markers (IL-6, IL-1β, TNF-α, and nitrite), and dopamine levels. Behavioral evaluation and rat brain histopathology were carried out to further support the neuroprotection.
Analysis using GC-MS revealed 36 constituents in KT. In vitro, the KT displayed considerable neuroprotective effects in terms of decreasing oxidative stress (ROS and SOD), neuroinflammation (IL-6, IL-1β, TNF-α, and nitrite), and elevating dopamine concentration. In vivo data showing improvements in histopathological and biochemical parameters confirmed the in vitro study findings, and in terms of behavioral assays, KT displayed significant activity.
GC-MS profiling was used to identify the bioactive compounds of KT with antioxidant, anti-inflammatory, and neuroprotective properties. As a result, they may be responsible for the therapeutic effects of KT on PD.
“Karkataka Taila (KT)，一种古老的阿育吠陀 Rasayana，由食用淡水蟹 Scylla serrata Forskal 的肉组成，仍然被喀拉拉邦的当地传统从业者用于治疗震颤和麻痹。在科学界，由于缺乏充分的科学验证和简短的报告，它的曝光度降低了。目前还没有关于 KT 在治疗帕金森病 (PD) 方面有效性的研究。
本研究的目的是研究 KT 对鱼藤酮诱导的 SH-SY5Y 细胞系和 PD 大鼠模型神经毒性的潜在抗帕金森作用，并探讨其潜在的分子机制。
通过气相色谱-质谱联用 (GC-MS) 鉴定 KT 的成分。使用 SH-SY5Y 细胞系和大鼠评估 KT 对鱼藤酮诱导的 PD 的神经保护作用。用于评估神经保护的参数包括抗氧化标志物 (ROS 和 SOD)、抗炎标志物 (IL-6、IL-1β、TNF-α 和亚硝酸盐) 和多巴胺水平。进行行为评估和大鼠脑组织病理学检查，以进一步支持神经保护作用。
GC-MS 分析显示 KT 中有 36 种成分。体外，KT 在降低氧化应激 (ROS 和 SOD)、神经炎症 (IL-6、IL-1β、TNF-α 和亚硝酸盐) 和提高多巴胺浓度方面表现出相当大的神经保护作用。体内数据显示组织病理学和生化参数的改善证实了体外研究的发现，并且在行为测定方面，KT 表现出显著的活性。
GC-MS 分析用于鉴定 KT 的具有抗氧化、抗炎和神经保护特性的生物活性化合物。因此，它们可能是 KT 治疗 PD 的治疗作用的原因。
