Sun W, Lin Z H, Wang H, Jia H, Tong L G, Zhang Z P, Li W, Zhou C C, Liu H
Medical School, Nantong University, Nantong 226001, China Affiliated Hospital of Nantong University, Nantong 226001, China.
Affiliated Hospital of Nantong University, Nantong 226001, China.
Zhonghua Xue Ye Xue Za Zhi. 2024 Jun 14;45(6):594-598. doi: 10.3760/cma.j.cn121090-20230927-00142.
To investigate the dynamic homing process and characteristics of macrophages in different organs of immune-mediated aplastic anemia (AA) model mice. Macrophages in donor lymph nodes were sorted by magnetic beads and labeled with PKH67. After modeling according to the preparation method of the AA model, peripheral blood rountine analysis, bone marrow biopsy and HE staining results were analyzed to verify the modeling effect. On days 4, 8, and 12 of modeling, the bone marrow, spleen, and lymph node mononuclear cells were collected, and dynamic changes of PKH67-labeled macrophages in donor mice were analyzed by flow cytometry. In this study, dynamic changes in PKH67-labeled macrophages in the pathogenesis of AA model mice were explored. Macrophages in donor mice homed to the lymph nodes, expanding and differentiating in the lymph nodes, and finally transported to the bone marrow and spleen. Through proteomics mass spectrometry analysis, the related immune inflammatory response pathway of macrophages involved in the activation of the AA bone marrow microenvironment was preliminarily revealed, which provides a basis for the pathological macrophages involved in the pathogenesis of AA model mice.
探讨免疫介导的再生障碍性贫血（AA）模型小鼠不同器官中巨噬细胞的动态归巢过程及特征。通过磁珠分选供体淋巴结中的巨噬细胞并用PKH67进行标记。按照AA模型制备方法建模后，分析外周血血常规、骨髓活检及HE染色结果以验证建模效果。在建模第4、8和12天，收集骨髓、脾脏和淋巴结单个核细胞，采用流式细胞术分析供体小鼠中PKH67标记的巨噬细胞的动态变化。本研究探索了AA模型小鼠发病过程中PKH67标记的巨噬细胞的动态变化。供体小鼠中的巨噬细胞归巢至淋巴结，在淋巴结中扩增并分化，最终转运至骨髓和脾脏。通过蛋白质组学质谱分析，初步揭示了参与激活AA骨髓微环境的巨噬细胞相关免疫炎症反应通路，为参与AA模型小鼠发病机制的病理性巨噬细胞提供了依据。
