抗大鼠肾脏肾素特异性高滴度抗体的制备。

Preparation of a specific high-titer antibody against rat kidney renin.

作者信息

Figueiredo A F, Inagami T

出版信息

Braz J Med Biol Res. 1985;18(2):151-5.

Abstract

Specific high-titer antibodies to rat kidney renin were raised using pure rat kidney renin conjugated with tetanus toxoid as antigen. The titers of two antisera for 50% inhibition of rat kidney renin activity were 80 000 and 700 000, respectively. The antibody inhibited the enzyme activities of hog kidney renin and mouse submaxillary gland renin at concentrations 1 000 times higher than required for rat kidney renin but did not inhibit human renal renin or rat kidney cathepsin D. The antibody can be used for characterizing the physiological and pathophysiological role of renin in blood pressure regulation, for the histochemical localization of renin in rat tissues and for distinguishing specific renin activity from the nonspecific renin-like activity of cathepsin D.

摘要

以与破伤风类毒素偶联的纯大鼠肾素作为抗原，制备了针对大鼠肾素的特异性高效价抗体。两种抗血清对大鼠肾素活性50%抑制的效价分别为80000和700000。该抗体在比抑制大鼠肾素所需浓度高1000倍的情况下，能抑制猪肾素和小鼠颌下腺肾素的酶活性，但不抑制人肾素或大鼠肾组织组织蛋白酶D。该抗体可用于表征肾素在血压调节中的生理和病理生理作用，用于肾素在大鼠组织中的组织化学定位，以及用于区分组织蛋白酶D的非特异性类肾素活性与特异性肾素活性。