Division of Molecular and Cellular Function, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
Methods Mol Biol. 2024;2831:97-111. doi: 10.1007/978-1-0716-3969-6_8.
To investigate the cell behavior underlying neuronal differentiation in a physiologically relevant context, differentiating neurons must be studied in their native tissue environment. Here, we describe an accessible protocol for fluorescent live imaging of differentiating neurons within ex vivo embryonic chicken spinal cord slice cultures, which facilitates long-term observation of individual cells within developing tissue.
为了在生理相关的背景下研究神经元分化的细胞行为，必须在其天然组织环境中研究分化的神经元。在这里，我们描述了一种可用于在体外鸡胚胎脊髓切片培养物中对分化神经元进行荧光实时成像的简单易行的方案，该方案有利于对发育组织内的单个细胞进行长期观察。
