胚胎鸡脊髓的卵内电穿孔与体外切片培养相结合，用于脊椎动物神经元分化的活体成像。

In Ovo Electroporation of Embryonic Chicken Spinal Cord in Combination with Ex Vivo Slice Culture for Live Imaging of Vertebrate Neuronal Differentiation.

机构信息

Division of Molecular and Cellular Function, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.

出版信息

Methods Mol Biol. 2024;2831:97-111. doi: 10.1007/978-1-0716-3969-6_8.

DOI:10.1007/978-1-0716-3969-6_8

PMID:39134846

Abstract

To investigate the cell behavior underlying neuronal differentiation in a physiologically relevant context, differentiating neurons must be studied in their native tissue environment. Here, we describe an accessible protocol for fluorescent live imaging of differentiating neurons within ex vivo embryonic chicken spinal cord slice cultures, which facilitates long-term observation of individual cells within developing tissue.

摘要

为了在生理相关的背景下研究神经元分化的细胞行为，必须在其天然组织环境中研究分化的神经元。在这里，我们描述了一种可用于在体外鸡胚胎脊髓切片培养物中对分化神经元进行荧光实时成像的简单易行的方案，该方案有利于对发育组织内的单个细胞进行长期观察。