Baljinnyam Tuvshintugs, Fukuda Satoshi, Niimi Yosuke, Prough Donald, Enkhbaatar Perenlei
Department of Anesthesiology, The University of Texas Medical Branch, Galveston, TX, USA.
Department of Pharmacology and Toxicology, The University of Texas Medical Branch, Galveston, TX, USA.
Lab Anim Res. 2024 Aug 12;40(1):27. doi: 10.1186/s42826-024-00213-7.
Sepsis is associated with a highest mortality rate in the ICU. Present study tests the efficacy of combined therapy with vitamin C, hydrocortisone and thiamine (combined therapy) in the ovine model of sepsis induced by Pseudomonas aeruginosa. In this study, sepsis was induced in sheep by instillation of Pseudomonas aeruginosa (1 × 10 CFU) into the lungs via bronchoscope, under anesthesia. Nine hours after injury, intravenous infusion of vitamin C (0.75 g every 6 h), hydrocortisone (25 mg every 6 h), and thiamine (100 mg every 12 h) or saline was given to the treatment and control groups. Cardiopulmonary variables were recorded.
The survival rate was 16.7% in control and 33.3% in treatment groups. In the control group, mean arterial pressure dropped from 93.6 ± 8.6 to 75.5 ± 9.7 mmHg by 9 h, which was not affected by the combined therapy. Pulmonary dysfunction was not attenuated by the combined therapy either. The combined therapy had no effect on increased extravascular lung water content and fluid effusion into thoracic cavity. The bacterial number in the bronchoalveolar lavage fluid was significantly increased in the treatment group than the control group. The blood bacterial number remained comparable between groups.
Combined vitamin C, hydrocortisone, and thiamine did not attenuate severity of ovine sepsis.
脓毒症在重症监护病房（ICU）中死亡率最高。本研究在铜绿假单胞菌诱导的绵羊脓毒症模型中测试维生素C、氢化可的松和硫胺联合治疗（联合治疗）的疗效。在本研究中，在麻醉状态下通过支气管镜将铜绿假单胞菌（1×10⁶CFU）注入绵羊肺部以诱导脓毒症。损伤9小时后，治疗组和对照组分别静脉输注维生素C（每6小时0.75g）、氢化可的松（每6小时25mg）、硫胺（每12小时100mg）或生理盐水。记录心肺变量。
对照组生存率为16.7%，治疗组为33.3%。对照组平均动脉压在9小时内从93.6±8.6降至75.5±9.7mmHg，联合治疗对此无影响。联合治疗也未减轻肺功能障碍。联合治疗对血管外肺水含量增加和胸腔积液无影响。治疗组支气管肺泡灌洗液中的细菌数量显著高于对照组。两组血中细菌数量相当。
维生素C、氢化可的松和硫胺联合治疗并未减轻绵羊脓毒症的严重程度。
