Cheung Tsz Lam, Ju Zhijie, Zhang Wenchao, Parker David, Deng Renren
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong 999077, China.
State Key Laboratory of Silicon and Advanced Semiconductor Materials, Institute for Composites Science Innovation, School of Materials Science and Engineering, Zhejiang University, Hangzhou 310058, China.
ACS Appl Mater Interfaces. 2024 Aug 21;16(33):43933-43941. doi: 10.1021/acsami.4c06899. Epub 2024 Aug 13.
Fluorescent nanothermometers based on thermal-dependent lifetime have a significant advantage in biological imaging owing to their immunity toward scattering, absorption, and autofluorescence. In this study, we present the first example of a water-soluble europium complex () that exhibits high sensitivity (1.2% K at 298 K) based on a temperature-dependent lifetime in the millisecond time range. This complex and its analogues show considerable potential for organelle imaging. The mechanism behind this thermal-sensitive behavior has been extensively investigated using transient absorption spectroscopy and variable temperature time-resolved luminescence methods. A highly efficient ligand sensitization process and a thermally activated back energy transfer process have been demonstrated. This study bridges the gap in small molecule thermometers with lifetimes longer than 1 ms and provides guidance in ligand design for metal coordination complex thermometers.
基于热依赖寿命的荧光纳米温度计在生物成像方面具有显著优势，因为它们对散射、吸收和自发荧光具有免疫性。在本研究中，我们展示了首例水溶性铕配合物（），其基于毫秒时间范围内的温度依赖寿命表现出高灵敏度（在298 K时为1.2% K）。该配合物及其类似物在细胞器成像方面显示出相当大的潜力。已使用瞬态吸收光谱和变温时间分辨发光方法对这种热敏行为背后的机制进行了广泛研究。已证明存在高效的配体敏化过程和热激活的反向能量转移过程。本研究填补了寿命超过1 ms的小分子温度计的空白，并为金属配位配合物温度计的配体设计提供了指导。
