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驱动荧光团在野生型和突变型纤毛神经元中差异表达。

driven fluorophores are differentially expressed in and mutant ciliated neurons.

作者信息

Power Kaiden M, Barr Maureen M

机构信息

Department of Genetics and Human Genetics Institute of New Jersey, Rutgers, The State University of New Jersey, New Brunswick, New Jersey, United States.

出版信息

MicroPubl Biol. 2024 Jul 25;2024. doi: 10.17912/micropub.biology.001245. eCollection 2024.

Abstract

Intraflagellar transport (IFT) involves the coordinated transport of molecular motors and other proteins and is required for ciliogenesis and ciliary maintenance. The IFT protein OSM-5 /IFT88 is expressed in a majority of the ciliated neurons in the animal, and mutants exhibit structurally defective cilia. The promoter is commonly used to express genetic constructs in the ciliated neurons. In this study, we show that brightness of driven constructs is altered in mutants of the tubulin deglutamylase and the NIMA-related kinase . This raises the possibility that expression levels may be regulated by and .

摘要

鞭毛内运输(IFT)涉及分子马达和其他蛋白质的协同运输,是纤毛发生和纤毛维持所必需的。IFT蛋白OSM-5/IFT88在动物体内大多数有纤毛的神经元中表达,突变体表现出结构有缺陷的纤毛。该启动子通常用于在有纤毛的神经元中表达基因构建体。在本研究中,我们表明,在微管蛋白去谷氨酰胺酶和NIMA相关激酶的突变体中,由该启动子驱动的构建体的亮度发生了改变。这增加了一种可能性,即该启动子的表达水平可能受上述两种酶的调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d536/11318990/b98d00a67045/25789430-2024-micropub.biology.001245.jpg

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