Vasileva Martina H, Bennemann Anette, Zachmann Karolin, Schön Michael P, Frank Jorge, Ulaganathan Vijay Kumar
Department of Dermatology, Venereology and Allergology, University Medical Center Göttingen, Göttingen, Germany.
Lower Saxony Institute of Occupational Dermatology, University Medical Center Göttingen, Göttingen, Germany.
Apoptosis. 2025 Feb;30(1-2):1-15. doi: 10.1007/s10495-024-01990-1. Epub 2024 Aug 13.
Anastasis is a phenomenon observed in cancer cells, where cells that have initiated apoptosis are able to recover and survive. This molecular event is increasingly recognized as a potential contributor to cancer metastasis, facilitating the survival and migration of tumor cells. Nevertheless, the identification of a specific surface marker for detecting cancer cells in anastasis remained elusive. Here we report our observation that the cell surface expression of CD24 is preferentially enriched in a non-adherent FSCSSC melanoma subpopulation, which is generally considered a non-viable population in cultivated melanoma cell lines. More than 90% of non-adherent FSCSSCCD24 metastatic melanoma cells exhibited bonafide features of apoptosis on the cell surface and in the nucleus, marking apoptotic or seemingly apoptotic subpopulations of the in vitro cultivated metastatic melanoma cell lines. Unexpectedly, however, the CD24 subpopulation, despite being apoptotic, showed evidence of metabolic activity and exhibited proliferative capacities, including anchorage-independent growth, when inoculated in soft agarose growth medium. These findings indicate that apoptotic FSCSSCCD24 melanoma subpopulations are capable of reversing the progression of apoptosis. We report CD24 as the first novel cell surface marker for anastasis in melanoma cells.
回生是在癌细胞中观察到的一种现象，即已经启动凋亡的细胞能够恢复并存活。这种分子事件越来越被认为是癌症转移的一个潜在促成因素，促进肿瘤细胞的存活和迁移。然而，用于检测处于回生状态的癌细胞的特异性表面标志物仍然难以确定。在此，我们报告我们的观察结果：CD24的细胞表面表达在非贴壁的FSC⁺SSC⁻黑色素瘤亚群中优先富集，该亚群在培养的黑色素瘤细胞系中通常被认为是无活力的群体。超过90%的非贴壁FSC⁺SSC⁻CD24⁺转移性黑色素瘤细胞在细胞表面和细胞核中表现出真正的凋亡特征，标志着体外培养的转移性黑色素瘤细胞系的凋亡或看似凋亡的亚群。然而，出乎意料的是，尽管CD24亚群处于凋亡状态，但当接种在软琼脂糖生长培养基中时，它显示出代谢活性的证据，并表现出增殖能力，包括不依赖贴壁生长。这些发现表明，凋亡的FSC⁺SSC⁻CD24⁺黑色素瘤亚群能够逆转凋亡进程。我们报告CD24是黑色素瘤细胞中首个用于回生的新型细胞表面标志物。
