ECO-Environment Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Shanghai Environmental Protection Monitoring Station of Agriculture, Shanghai 201403, China; Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China; Shang Hai Rightway Environmental Protection Technology Co.，Ltd, Shanghai 200131, China.
ECO-Environment Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
Ecotoxicol Environ Saf. 2024 Sep 15;283:116870. doi: 10.1016/j.ecoenv.2024.116870. Epub 2024 Aug 13.
The biogeochemical processes of sulfur and heavy metals in the environment are closely related to each other. We investigated the influence of sulfur addition on hyperaccumulator Sedum alfredii Hance growth, cadmium (Cd) accumulation, soil Cd bioavailability, soil bacterial communities and plant transcriptome responses. The results showed that an appropriate rate of sulfur addition (1.0 or 2.5 g/kg) enhanced the growth of Sedum alfredii Hance plants as well as their accumulation of Cd. A high rate of sulfur addition (5.0 or 10.0 g/kg) causes toxicity to Sedum alfredii Hance plants. The application of an appropriate amount of sulfur to the soil increased the abundance of sulfur-oxidizing bacteria such as Sulfuriferula and Thiobacillus; acid-fast bacillus such as Alicyclobacillus; and cadmium-tolerant bacteria such as Bacillus and Rhodanobacter. This led to a decrease in pH and an increase in bioavailable Cd in the soil. RNA sequencing revealed that the addition of sulfur to soils led to the up regulation of most of the differentially expressed genes (DEGs) involved in "photosynthesis" and "photosynthesis, light reaction" in Sedum alfredii Hance leaves. Moreover, the "plant hormone signal transduction" pathway was significantly enriched with sulfur addition. Sulfur assimilation in Sedum alfredii Hance plants may promote photosynthesis and hormone synthesis, leading to Cd tolerance in these plants. Our study revealed that sulfur fertilization enhanced the efficiency of Cd phytoremediation in Sedum alfredii Hance plants.
环境中硫和重金属的生物地球化学过程密切相关。我们研究了添加硫对超积累植物垂盆草生长、镉（Cd）积累、土壤 Cd 生物有效性、土壤细菌群落和植物转录组响应的影响。结果表明，适量的硫添加（1.0 或 2.5 g/kg）可增强垂盆草植物的生长及其对 Cd 的积累。高浓度的硫添加（5.0 或 10.0 g/kg）会对垂盆草植物造成毒性。适量的硫应用于土壤中会增加硫氧化菌（如 Sulfuriferula 和 Thiobacillus）、耐酸菌（如 Alicyclobacillus）和耐镉菌（如 Bacillus 和 Rhodanobacter）的丰度。这导致土壤 pH 值降低，生物可利用 Cd 增加。RNA 测序表明，土壤中添加硫会导致垂盆草叶片中与“光合作用”和“光合作用、光反应”相关的大多数差异表达基因（DEGs）上调。此外，“植物激素信号转导”途径在硫添加时显著富集。垂盆草植物中硫的同化可能会促进光合作用和激素合成，从而提高这些植物对 Cd 的耐受性。我们的研究表明，硫施肥增强了垂盆草植物对 Cd 的植物修复效率。
