Lenn N J, Leranth C, Zaborszky L
J Neurocytol. 1985 Dec;14(6):909-19. doi: 10.1007/BF01224804.
The cholinergic synapses of the rat interpeduncular nucleus (IPN) were demonstrated by immunostaining that utilized a monoclonal antibody directed against choline acetyltransferase. The rostral, central, intermediate and lateral subnuclei of the IPN each contained a single type of immunoreactive terminal. Immunoreactivity was localized to synaptic vesicle membranes (especially at the contact zones), and longitudinal microtubules in preterminal portions of axons. Terminals were identified by comparison to previous studies of the synaptic organization of the IPN. In the rostral subnucleus, the immunoreactive terminals were characterized by their content of spherical vesicles, 45 nm in diameter, intermixed with moderate numbers of dense-cored vesicles, 75-100 nm in diameter. These terminals formed asymmetrical contacts. They correspond to the more numerous of the two types of axodendritic terminals described in this subnucleus, i.e. those which degenerate after lesions of the habenula. The moderate number of immunoreactive terminals in the lateral subnucleus contained pleomorphic vesicles, 30-45 nm in diameter. Up to three of these formed symmetrical contacts with individual dendrites, which ranged in diameter from 0.35 to 0.55 micron. The other types of axodendritic terminal in this subnucleus, which often contacted the same dendrites, were unstained. These latter terminals have been interpreted as being those which contain substance P. The immunoreactive terminals in the diameter, and formed markedly asymmetrical en passant contacts with small dendritic processes or spines. The immunoreactive terminals in the intermediate subnucleus had the same presynaptic and contact morphology. Many were clearly crest synapses. The remainder appeared to be such, but seen only partially within the plane of section. In the intermediate subnucleus there were up to several hundred immunostained terminals per grid square in some sections. These findings are consistent with the existence of a dense cholinergic projection to the IPN. The habenular region, are shown to crest and S synapses, both of which generate after lesions of the cholinergic innervation of other subnuclei of the IPN increases understanding of the relation of cholinergic to other transmitters localized to various portions of this nucleus.
利用针对胆碱乙酰转移酶的单克隆抗体进行免疫染色，证实了大鼠脚间核（IPN）的胆碱能突触。IPN的嘴侧、中央、中间和外侧亚核各自包含单一类型的免疫反应性终末。免疫反应性定位于突触小泡膜（尤其是在接触区）以及轴突终末前部分的纵向微管。通过与先前关于IPN突触组织的研究进行比较来识别终末。在嘴侧亚核中，免疫反应性终末的特征在于其含有直径为45nm的球形小泡，与中等数量的直径为75 - 100nm的有致密核心的小泡混合。这些终末形成不对称接触。它们对应于该亚核中描述的两种类型的轴 - 树突终末中数量较多的那种，即那些在缰核损伤后退化的终末。外侧亚核中数量适中的免疫反应性终末含有直径为30 - 45nm的多形性小泡。其中多达三个与直径为0.35至0.55微米的单个树突形成对称接触。该亚核中其他类型的轴 - 树突终末（它们经常接触相同的树突）未被染色。后者这些终末被解释为含有P物质的终末。中间亚核中的免疫反应性终末具有相同的突触前和接触形态。许多明显是嵴突触。其余的似乎是，但仅在切片平面内部分可见。在中间亚核的一些切片中，每个网格正方形中有多达数百个免疫染色终末。这些发现与存在向IPN的密集胆碱能投射一致。缰核区域显示有嵴突触和S突触，这两种突触在IPN其他亚核的胆碱能神经支配损伤后都会产生，这增加了对胆碱能与定位于该核不同部分的其他递质之间关系的理解。
