Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Toxicology. 2024 Nov;508:153920. doi: 10.1016/j.tox.2024.153920. Epub 2024 Aug 11.
Mycotoxins have strong immunotoxicity and can induce oxidative stress and mitochondrial dynamics imbalance. Mitochondrial antiviral signaling protein (MAVS) in the RIG-I like receptor (RLR) pathway of innate immunity is located on mitochondria, and whether it is affected by mycotoxins has not been reported yet. This experiment used porcine alveolar macrophages (PAM) to evaluate the antagonism of three isomers of chlorogenic acid (chlorogenic acid, isochlorogenic acid A, and neochlorogenic acid) against combined mycotoxins (Aflatoxin B1, Deoxynivalenol, and Ochratoxin A) induced mitochondrial damage and their effects on the RLR pathway, providing assistance for further elucidating the mechanism of mycotoxin immunotoxicity. Western blotting, enzyme linked immunosorbent assay (ELISA), and flow cytometry were used to detect relevant indicators. All three types of chlorogenic acid treatment can antagonize the cytotoxicity induced by combined mycotoxins, especially isochlorogenic acid A, which can protect cells from mycotoxins damage by maintaining mitochondrial dynamic homeostasis and improving innate immune function related to the RLR pathway.
真菌毒素具有很强的免疫毒性，可诱导氧化应激和线粒体动力学失衡。先天免疫中 RIG-I 样受体 (RLR) 途径中的线粒体抗病毒信号蛋白 (MAVS) 位于线粒体上，但其是否受真菌毒素影响尚未有报道。本实验采用猪肺泡巨噬细胞（PAM）评估 3 种绿原酸异构体（绿原酸、异绿原酸 A 和新绿原酸）对联合真菌毒素（黄曲霉毒素 B1、脱氧雪腐镰刀菌烯醇和赭曲霉毒素 A）诱导的线粒体损伤的拮抗作用及其对 RLR 途径的影响，为进一步阐明真菌毒素免疫毒性的机制提供帮助。Western blot、酶联免疫吸附测定（ELISA）和流式细胞术用于检测相关指标。所有 3 种类型的绿原酸处理都能拮抗联合真菌毒素诱导的细胞毒性，特别是异绿原酸 A，它通过维持线粒体动力学平衡和改善与 RLR 途径相关的先天免疫功能来保护细胞免受真菌毒素的损伤。
