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木瓜蛋白酶添加到真空低温烹调对腌制半腱子牛肉牛排的质地和感官特性的影响。

Impact of adding papain to sous vide cooking on texture and sensorial traits of marinated semitendinosus beefsteaks.

机构信息

Departamento de Ciência dos Alimentos, Escola de Ciências Agrárias de Lavras, Universidade Federal de Lavras (UFLA), Lavras, Minas Gerais, Brazil.

出版信息

J Texture Stud. 2024 Aug;55(4):e12860. doi: 10.1111/jtxs.12860.

DOI:10.1111/jtxs.12860
PMID:39138115
Abstract

Sous vide meat is an emerging food category, the consumption of which has increased owing to greater convenience, sensory traits, elderly consumers acceptance, and low-cost cuts use. However, required prolonged thermal treatment to achieve desired tenderness, impact energy-consumption besides triggering lipid oxidation, undesired off-flavors, and cooked meat profiles. Using a response surface methodology (RSM), this study evaluated the effects of the vegetal proteolytic papain (0 to 20 mg/kg) and low-temperature sous vide cooking (SVC) time (1 to 8 h at 65°C) in low-value marinated M. semitendinosus beefsteaks on technological characteristics associated with tenderness, and lipid oxidation. Additionally, the sensory profile traits of the pre-selected treatments were described using check-all-that-apply (CATA) and preference mapping. Shear force (WBsSF) was reduced with greater papain addition, whereas higher cooking losses (CL) were observed with longer SVC cooking times. Both the released total collagen and TBARS values increased with increasing papain concentrations and SVC times. Combining high levels of papain (>10 mg/kg) and SVC time (>6 h) resulted in lower WBsSF values (<20 N) but higher CL (>27%) and the CATA descriptors "aftertaste" and "mushy." The optimized conditions (14 mg/kg papain; 2 h SVC) also reduced WBsSF values (<26 N) with lower CL (<20%) and were most preferred and described as "juicy" and "tender" by consumers. Observed results suggest that combined mild SVC and papain may potentiate tenderness, conjointly favor juiciness and oxidation, further representing a promising tool for reducing SVC time without compromising valued sous vide sensory traits.

摘要

低温慢煮肉是一种新兴的食品类别,由于其更加方便、具有感官特性、老年人易于接受以及能够使用低成本切割肉,其消费有所增加。然而,为了达到理想的嫩度,需要进行长时间的热处理,这不仅会增加能耗,还会引发脂质氧化、产生不良风味和烹饪后肉的质地变差。本研究采用响应面法(RSM),评估了植物蛋白酶木瓜蛋白酶(0 至 20mg/kg)和低温慢煮(SVC)时间(65°C 下 1 至 8 小时)对腌制低价值半腱肌牛肉牛排嫩度相关技术特性和脂质氧化的影响。此外,还使用可复选(CATA)和偏好映射描述了预选处理的感官特征。随着木瓜蛋白酶添加量的增加,剪切力(WBSSF)降低,而 SVC 烹饪时间越长,烹饪损失(CL)越高。随着木瓜蛋白酶浓度和 SVC 时间的增加,释放的总胶原蛋白和 TBARS 值均增加。高浓度的木瓜蛋白酶(>10mg/kg)和 SVC 时间(>6 小时)的组合导致较低的 WBSF 值(<20N),但较高的 CL(>27%)和 CATA 描述符“余味”和“糊状”。优化条件(14mg/kg 木瓜蛋白酶;2h SVC)还降低了 WBSF 值(<26N),CL 值较低(<20%),且消费者最偏好并描述为“多汁”和“嫩”。观察结果表明,温和的 SVC 和木瓜蛋白酶的组合可以增强嫩度,共同有利于多汁性和氧化,进一步代表了一种有前途的工具,可以在不影响低温慢煮感官特性的情况下缩短 SVC 时间。

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