Esrig S, Rubinstein L J, Goldstein B
Mol Immunol. 1985 Dec;22(12):1323-32. doi: 10.1016/0161-5890(85)90053-7.
We studied the time development of direct hemolytic plaques in thin layers containing either sheep red blood cells (SRBC) directly haptenated (DH) with trinitrophenyl, or SRBC indirectly haptenated (IH) with dinitrophenyl coupled to human serum albumin. The DH-SRBC tend to be sparsely haptenated while the IH-SRBC tend to have very high local hapten densities. We observed marked differences in the growth of plaques for the two differently haptenated SRBC. Plots of the plaque radius squared vs time show that the slope of those curves that developed in a lawn of DH-SRBC tended to be constant while the slope of those curves that developed in a lawn of IH-SRBC tended to decrease with time. These results are what is predicted from theory if: IgM binds to DH-SRBC through attachments that rapidly dissociate, if IgM binds to IH-SRBC through attachments that very slowly dissociate, and if (3) both types of bound IgM can fix and activate complement.
我们研究了在含有直接用三硝基苯半抗原化的绵羊红细胞（SRBC）（DH）或用与人血清白蛋白偶联的二硝基苯间接半抗原化的SRBC（IH）的薄层中直接溶血斑的时间发展情况。DH-SRBC往往半抗原化程度稀疏，而IH-SRBC往往具有非常高的局部半抗原密度。我们观察到两种不同半抗原化的SRBC在噬斑生长方面存在显著差异。噬斑半径平方对时间的绘图表明，在DH-SRBC菌苔中形成的曲线斜率趋于恒定，而在IH-SRBC菌苔中形成的曲线斜率趋于随时间降低。如果：IgM通过快速解离的结合物与DH-SRBC结合，IgM通过非常缓慢解离的结合物与IH-SRBC结合，并且（3）两种结合的IgM都能固定并激活补体，那么这些结果就是理论所预测的。
