Centre for Future Foods, Auckland University of Technology, Auckland, New Zealand.
J Food Sci. 2024 Sep;89(9):5395-5410. doi: 10.1111/1750-3841.17277. Epub 2024 Aug 13.
This study investigated the use of microencapsulated Asiatic pennywort (Centella asiatica) (CA) as a functional ingredient to formulate a novel chocolate oat milk beverage. The main objectives of the study were to characterize and encapsulate bioactive components from CA and to determine the polyphenol content and sensory properties of the beverage. CA extract was microencapsulated using maltodextrin and gum Arabic as carriers and subsequently freeze-dried to produce microcapsules. Microencapsulated CA was incorporated into chocolate oat milk at varying concentrations. Polyphenol content of the beverages was quantified using liquid chromatography-mass spectrometry. Consumer acceptability and sensory perception of the beverages were evaluated through an acceptance test and a check-all-that-apply test, respectively, to assess the sensory characteristics of the chocolate oat milk beverage. CA fortified chocolate oat milk contained fourteen polyphenols. Increasing the concentration of microencapsulated CA led to an increase in the polyphenol content of the beverage. Among the identified polyphenols, asiatic acid and asiaticoside stood out as the unique and most abundant compounds in CA (p < 0.05). Additionally, the incorporation of cocoa powder into the beverage further contributed to the polyphenol content, introducing bioactive compounds such as benzoic acid, caffeic acid, catechin, chlorogenic acid, kaempferol, luteolin, madecassic acid, p-coumaric acid, and quercetin. Evaluation of consumer acceptability revealed that chocolate oat milk beverages containing 2% and 4% microencapsulated CA were liked by consumers. However, beverages with higher concentrations of CA were perceived as less acceptable, characterized by grassy, bitter, and earthy attributes. In conclusion, this study demonstrates the potential of microencapsulated CA as a functional ingredient in chocolate oat milk beverages. PRACTICAL APPLICATION: This study reveals new insights on the microencapsulation of bioactive compounds in CA, proposing its potential as a novel functional ingredient in food and beverage applications in Western markets. The study revealed microencapsulated CA retained polyphenols in CA including asiatic acid and asiaticoside responsible for its bioactive properties. Consumer perception of CA added to oat milk revealed that it can be added at an acceptable level of 4%; however, higher amounts can decrease consumer acceptability. As practitioners explore the incorporation of CA as a functional component in food products, it is crucial to explore preservation techniques for the sensitive bioactive components while balancing the optimal amount of CA to enhance overall consumer liking.
本研究旨在探讨将微囊化积雪草(Centella asiatica)(CA)用作功能性成分,以开发新型巧克力燕麦奶饮料。本研究的主要目的是对 CA 的生物活性成分进行特征描述和包埋,并测定饮料的多酚含量和感官特性。CA 提取物用麦芽糊精和阿拉伯胶作为载体进行微囊化,然后进行冷冻干燥以生产微胶囊。将微囊化 CA 以不同浓度添加到巧克力燕麦奶中。使用液相色谱-质谱法定量测定饮料中的多酚含量。通过接受测试和勾选所有适用项测试分别评估消费者对饮料的可接受性和感官感知,以评估巧克力燕麦奶饮料的感官特性。CA 强化巧克力燕麦奶含有 14 种多酚。随着微囊化 CA 浓度的增加,饮料中的多酚含量也随之增加。在所鉴定的多酚中,积雪草酸和积雪草苷是 CA 中含量最高的独特化合物(p < 0.05)。此外,将可可粉加入饮料中也进一步增加了多酚含量,引入了生物活性化合物,如苯甲酸、咖啡酸、儿茶素、绿原酸、山奈酚、木樨草素、积雪草酸、对香豆酸和槲皮素。消费者接受度评估表明,含有 2%和 4%微囊化 CA 的巧克力燕麦奶饮料受到消费者的喜爱。然而,含有更高浓度 CA 的饮料被认为不太受欢迎,具有草味、苦味和土味等特征。总之,本研究表明微囊化 CA 作为巧克力燕麦奶饮料的功能性成分具有潜力。 实际应用:本研究揭示了 CA 中生物活性化合物微囊化的新见解,提出了其作为西方市场食品和饮料应用中新型功能性成分的潜力。研究表明,微囊化 CA 保留了 CA 中的多酚,包括积雪草酸和积雪草苷,这些成分赋予了 CA 生物活性。消费者对添加到燕麦奶中的 CA 的感知表明,它可以以可接受的 4%水平添加;然而,更高的添加量可能会降低消费者的接受度。由于从业者正在探索将 CA 作为功能性成分添加到食品产品中,因此在平衡 CA 的最佳添加量以提高整体消费者喜好的同时,探索对敏感生物活性成分的保存技术至关重要。