https://ror.org/04mkzax54 Science Center for Future Foods, Jiangnan University, Wuxi, China.
https://ror.org/04mkzax54 School of Bioengineering, Jiangnan University, Wuxi, China.
Life Sci Alliance. 2024 Apr 4;7(6). doi: 10.26508/lsa.202402606. Print 2024 Jun.
For establishing sister chromatid cohesion and proper chromosome segregation in mitosis in fission yeast, the acetyltransferase Eso1 plays a key role. Eso1 acetylates cohesin complexes, at two conserved lysine residues K105 and K106 of the cohesin subunit Psm3. Although Eso1 also contributes to reductional chromosome segregation in meiosis, the underlying molecular mechanisms have remained elusive. Here, we purified meiosis-specific Rec8 cohesin complexes localized at centromeres and identified a new acetylation at Psm3-K1013, which largely depends on the meiotic kinetochore factor meikin (Moa1). Our molecular genetic analyses indicate that Psm3-K1013 acetylation cooperates with canonical acetylation at Psm3-K105 and K106, and plays a crucial role in establishing reductional chromosome segregation in meiosis.
为了在裂殖酵母有丝分裂中建立姐妹染色单体黏合和正确的染色体分离，乙酰转移酶 Eso1 起着关键作用。Eso1 乙酰化黏合复合物，在黏合亚基 Psm3 的两个保守赖氨酸残基 K105 和 K106 上。尽管 Eso1 也有助于减数分裂中的消减染色体分离，但潜在的分子机制仍然难以捉摸。在这里，我们纯化了定位于着丝粒的减数分裂特异性 Rec8 黏合复合物，并鉴定了 Psm3-K1013 上的一个新的乙酰化，这在很大程度上依赖于减数分裂着丝粒因子 meikin（Moa1）。我们的分子遗传学分析表明，Psm3-K1013 乙酰化与 Psm3-K105 和 K106 的经典乙酰化协同作用，在减数分裂中建立消减染色体分离中起着至关重要的作用。
