Candy N G, Ramezanpour M, Bouras G, Chegeni N, Chataway T, Ormsby R J, Jukes A K, Vreugde S, Wormald P-J, Psaltis A J
Department of Surgery - Otolaryngology, Head and Neck Surgery, The University of Adelaide, Basil Hetzel Institute for Translational Research, Woodville South, Adelaide, Australia.
Flinders Proteomic Facility, Department of Human Physiology, Flinders University, Bedford Park, Australia.
Rhinology. 2024 Dec 1;62(6):750-758. doi: 10.4193/Rhin24.216.
Pituitary neuroendocrine tumours (PitNETs) are common accounting for 10 to 25 % of all intracranial tumours. This project describes the feasibility of developing a novel membrane-based biomarker that could be used for fluorescent guided surgery. The aim was to catalogue the differential expression of membrane proteins between non-functional PitNETs and pituitary glands.
Ten pituitary gland tissue specimens were obtained from the National Institute of Health (NIH) NeuroBio-Bank and twenty non-functional PitNETs were obtained from the Northwestern University Nervous System Tumour Bank. Mass spectrometry analysis using an Orbitrap Fusion Lumos Tribrid Mass Spectrometer linked to a Dionex Ultimate 3000 UPLC system was undertaken. Data Dependent Acquisition Mass Spectrometry and Data Independent Acquisition Mass Spectrometry was then completed. Pathway enrichment analysis was performed using clusterProfiler v4.6.0. Functional enrichment analysis was conducted using Gene Ontology terms and Reactome pathways.
Differential expression analysis between the two groups revealed a total of 2110 significant differently expressed proteins (DEPs), with 1387 of these also having a Log2 fold change either greater than 1, or less than -1. Of the 2110 DEPs, 925 were upregulated in tumours compared to control, while 1185 were downregulated.
We have demonstrated a proteomic comparison between non-functional PitNETs and normal pituitary glands. These results demonstrate differences consistent with contemporary literature but shows that NOTCH3 and PTPRJ are up-regulated in non-functional PitNETs compared to pituitary glands.
垂体神经内分泌肿瘤（PitNETs）很常见，占所有颅内肿瘤的10%至25%。本项目描述了开发一种可用于荧光引导手术的新型基于膜的生物标志物的可行性。目的是梳理无功能PitNETs与垂体之间膜蛋白的差异表达情况。
从美国国立卫生研究院（NIH）神经生物样本库获取10份垂体组织标本，从西北大学神经系统肿瘤样本库获取20份无功能PitNETs标本。使用与戴安UltiMate 3000超高效液相色谱系统联用的奥睿科三合一串联质谱仪进行质谱分析。然后完成数据依赖型采集质谱分析和数据非依赖型采集质谱分析。使用clusterProfiler v4.6.0进行通路富集分析。使用基因本体术语和Reactome通路进行功能富集分析。
两组之间的差异表达分析显示共有2110种显著差异表达蛋白（DEPs），其中1387种的Log2倍数变化大于1或小于-1。在这2110种DEPs中，与对照组相比，肿瘤中有925种上调，1185种下调。
我们展示了无功能PitNETs与正常垂体之间的蛋白质组学比较。这些结果显示出与当代文献一致的差异，但表明与垂体相比，NOTCH3和PTPRJ在无功能PitNETs中上调。
