Institute of Nanotechnology (INT), Karlsruhe Institute of Technology (KIT), Kaiserstraße 12, 76131, Karlsruhe, Germany.
Chemistry. 2024 Sep 2;30(49):e202401071. doi: 10.1002/chem.202401071. Epub 2024 Aug 14.
Polyamines are essential analytes due to their critical role in various biological processes and human health in general. Due to their role as regulators for cell growth and proliferation (putrescine and spermine), as neuroprotectors, gero-, and cardiovascular protectors (spermidine), and as bacterial growth indicators (cadaverine), rapid, simple, and cost-effective methods for polyamine detection in biofluids are in demand. The present study focuses on the development and investigation of self-assembled and fluorescent host⋅dye chemo-sensors based on sulfonated pillar[5]arene for the specific detection of polyamines. Binding studies, as well as stability and functionality assessments of the turn-on chemosensors for selective polyamine detection in saline and biologically relevant media, are shown. Furthermore, the practical applicability of the developed chemo-sensors is demonstrated in biofluids such as human urine and saliva.
多胺是重要的分析物，因为它们在各种生物过程和人类健康中起着关键作用。由于它们作为细胞生长和增殖的调节剂（腐胺和精胺）、神经保护剂、衰老和心血管保护剂（亚精胺）以及细菌生长指标（尸胺）的作用，因此需要快速、简单和具有成本效益的生物流体中多胺检测方法。本研究专注于基于磺化的[pillar[5]arene]的自组装和荧光主体-染料化学传感器的开发和研究，用于多胺的特异性检测。展示了结合研究以及对开启型化学传感器的稳定性和功能评估，用于在盐和生物相关介质中选择性检测多胺。此外，还在生物流体（如人尿和唾液）中证明了所开发的化学传感器的实际应用。
