序贯 ChIP-Seq。

Sequential ChIP-Seq.

机构信息

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Pittsburgh Liver Research Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

出版信息

Methods Mol Biol. 2024;2846:109-121. doi: 10.1007/978-1-0716-4071-5_7.

DOI:10.1007/978-1-0716-4071-5_7

PMID:39141232

Abstract

ChIP-Seq has been used extensively to profile genome-wide transcription factor binding and post-translational histone modifications. A sequential ChIP assay determines the in vivo co-localization of two proteins to the same genomic locus. In this chapter, we combine the two protocols in Sequential ChIP-Seq, a method for identifying genome-wide sites of in vivo protein co-occupancy.

摘要

ChIP-Seq 已被广泛用于绘制全基因组转录因子结合和翻译后组蛋白修饰图谱。顺序 ChIP 测定法可确定两种蛋白质在体内是否共定位到同一基因组位点。在本章中，我们将这两个方案结合起来，形成了一种用于鉴定体内蛋白质共占据的全基因组位点的方法，即顺序 ChIP-Seq。