Department of Molecular & Systems Biology, Dartmouth College, Lebanon, NH, USA.
Dartmouth Cancer Center, Dartmouth College, Lebanon, NH, USA.
Methods Mol Biol. 2024;2846:169-179. doi: 10.1007/978-1-0716-4071-5_11.
Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) allows for the identification of genomic targeting of DNA-binding proteins. Cleavage Under Targets and Release Using Nuclease (CUT&RUN) modifies this process by including a nuclease to digest DNA around a protein of interest. The result is a higher signal-to-noise ratio and decreased required starting material. This allows for high-fidelity sequence identification from as few as 500 cells, enabling chromatin profiling of precious tissue samples or primary cell types, as well as less abundant chromatin-binding proteins: all at significantly increased throughput.
染色质免疫沉淀测序（ChIP-Seq）可用于鉴定 DNA 结合蛋白的基因组靶标。通过在包含核酸酶的过程中对目标进行切割和释放（CUT&RUN）来修改该过程，该核酸酶可以消化靶蛋白周围的 DNA。其结果是信号与噪声的比值更高，所需起始材料减少。这使得即使从少至 500 个细胞中也能进行高保真度的序列鉴定，从而能够对珍贵的组织样本或原代细胞类型进行染色质分析，以及对较少丰度的染色质结合蛋白进行分析：所有这些都显著提高了通量。
