Institute of Diagnostic and Interventional Radiology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, PR China.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai 201620, PR China.
ACS Biomater Sci Eng. 2024 Oct 14;10(10):6483-6497. doi: 10.1021/acsbiomaterials.4c00925. Epub 2024 Aug 14.
Endovascular treatment (EVT) using stents has become the primary option for severe cerebrovascular stenosis. However, considerable challenges remain to be addressed, such as in-stent restenosis (ISR) and late thrombosis. Many modified stents have been developed to inhibit the hyperproliferation of vascular smooth muscle cells (SMCs) and protect vascular endothelial cells (VECs), thereby reducing such complications. Some modified stents, such as those infused with rapamycin, have improved in preventing acute thrombosis. However, ISR and late thrombosis, which are long-term complications, remain unavoidable. (PNS), a traditional Chinese medicine consisting of various compounds, is beneficial in promoting the proliferation and migration of VECs and inhibiting the proliferation of SMCs. Herein, a 3D-printed polycaprolactone (PCL) stent loaded with PNS (PNS-PCL stent) was developed based on a previous study. studies confirmed that PNS promotes the migration and proliferation of VECs, which were damaged, by increasing the expression levels of microRNA-126, p-AKT, and endothelial nitric oxide synthase. , the PNS-PCL stents maintained the patency of the carotid artery in rabbits for up to three months, outperforming the PCL stents. The PNS-PCL stents may present a new solution for the EVT of cerebrovascular atherosclerotic stenosis in the future.
血管内治疗 (EVT) 使用支架已成为严重脑血管狭窄的主要选择。然而，仍有许多挑战需要解决，如支架内再狭窄 (ISR) 和晚期血栓形成。许多改良的支架已被开发出来，以抑制血管平滑肌细胞 (SMCs) 的过度增殖并保护血管内皮细胞 (VECs)，从而减少这些并发症。一些改良的支架，如那些注入雷帕霉素的支架，已改善了预防急性血栓形成的效果。然而，ISR 和晚期血栓形成等长期并发症仍然不可避免。
（PNS），一种由多种化合物组成的中药，有利于促进 VEC 的增殖和迁移，并抑制 SMC 的增殖。在此基础上，基于之前的研究，开发了一种载有 PNS 的 3D 打印聚己内酯 (PCL) 支架 (PNS-PCL 支架)。研究证实，PNS 通过增加 microRNA-126、p-AKT 和内皮型一氧化氮合酶的表达水平，促进受损 VEC 的迁移和增殖。研究表明，在三个月的时间里，PNS-PCL 支架保持了兔颈动脉的通畅性，优于 PCL 支架。PNS-PCL 支架可能为未来的脑血管粥样硬化狭窄的 EVT 提供新的解决方案。
