School of Life and Environmental Sciences, Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, Guilin University of Electronic Technology, Guilin, Guangxi, 541004, China.
School of Life and Environmental Sciences, Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, Guilin University of Electronic Technology, Guilin, Guangxi, 541004, China.
Talanta. 2024 Dec 1;280:126677. doi: 10.1016/j.talanta.2024.126677. Epub 2024 Aug 6.
Taylor dispersion analysis (TDA) is a rapid and precise method for determining the hydrodynamic radius (R) of various substances. We present a versatile TDA system with a flow-through sample injection device, two compact 3-in-1 detectors, and a high-voltage power supply. The 3D-printed detectors combine fluorimetry (FD), photometry (AD@255 nm), and contactless conductometry (CD) in a single head, enabling simultaneous detection at one capillary window. Using bovine serum albumin (BSA) as a model analyte, we compare TDA with different detection methods. BSA labeled with fluorescein isothiocyanate (FITC) is analyzed in both pulse mode and capillary electrophoresis (CE) TDA. FD and AD detection yield similar R values, except when FITC binds with small ions in the buffer. In phosphate buffer, CD underestimates R values by approximately 18 % due to BSA self-association. In Tris-based buffers, CD values are 87%-96 % of AD values in pulse mode. With CE-TDA using Tris-CHES buffer, no statistical difference is found across all detections. The system is also applied to CE-TDA of various compounds, particularly charged saccharides. CE-TDA improves the accuracy of TDA results from CD. We demonstrate the resolution of mixed CD-TDA signals with assistance from FD and AD signals, successfully resolving gluconate peaks fully covered by another compound. The versatile system with 3-in-1 detection offers a powerful tool for TDA of mixtures and enhances sample throughput.
泰勒分散分析（TDA）是一种快速、精确的方法，用于测定各种物质的流体力学半径（R）。我们提出了一种多功能的 TDA 系统，该系统具有流通式样品注入装置、两个紧凑型 3-in-1 检测器和一个高压电源。3D 打印的检测器将荧光法（FD）、光度法（AD@255nm）和非接触电导法（CD）组合在一个探头中，可在一个毛细管窗口同时进行检测。我们使用牛血清白蛋白（BSA）作为模型分析物，比较了 TDA 与不同检测方法的结果。BSA 用异硫氰酸荧光素（FITC）标记后，在脉冲模式和毛细管电泳（CE）TDA 中进行分析。FD 和 AD 检测得到的 R 值相似，除了 FITC 与缓冲液中的小离子结合时。在磷酸盐缓冲液中，由于 BSA 自组装，CD 会低估 R 值约 18%。在基于 Tris 的缓冲液中，CD 值在脉冲模式下为 AD 值的 87%-96%。使用 Tris-CHES 缓冲液的 CE-TDA，所有检测结果均无统计学差异。该系统还应用于各种化合物的 CE-TDA，特别是带电的糖。CE-TDA 提高了 CD 对 TDA 结果的准确性。我们展示了 FD 和 AD 信号辅助下混合 CD-TDA 信号的分辨率，成功地完全分离了另一种化合物完全覆盖的葡萄糖酸盐峰。该多功能系统具有 3-in-1 检测功能，为混合物的 TDA 提供了强大的工具，并提高了样品通量。
