Zheng Jianbo, Yao Yuefei, Rui Qianlong, Zhou Yangda, Li Fei, Jiang Wenping, Chi Meili, Liu Shili, Cheng Shun, Chen Jianming, Wang Shu
Key Laboratory of Genetics and Breeding, Zhejiang Institute of Freshwater Fisheries, Huzhou, China; Huzhou academy of agricultural Science, China.
School of of marine science, Ningbo University, Ningbo, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2024 Dec;52:101301. doi: 10.1016/j.cbd.2024.101301. Epub 2024 Aug 8.
Mandarin fish (Siniperca chuatsi) represents a typical carnivorous freshwater economic fish in China. Recently, the study of their feeding behavior to acclimate formulated diets has become a research focus. This study evaluated the effects of various diets on the body composition, nutritional content, digestive enzyme activity, gene expression, and gut microbiota of mandarin fish. Firstly, no significant differences were found in the muscle's basic nutritional components (moisture, crude protein, crude fat, and crude ash), as well as in the fatty acid and amino acid content, between the live feed group (LFSC) and the compound feed group (CFSC). However, mandarin fish in the LFSC group exhibited significantly higher lipase activity in the liver and intestine compared to the CFSC group, while amylase activity in the intestine showed an opposite pattern. Additionally, intestinal transcriptome analysis revealed 6238 differentially expressed genes and identified several differentially expressed clock genes associated with diet type. Furthermore, gut microbiota analysis indicated that different feeding regimens influenced microbial composition, revealing correlations between bacterial genera and intestinal gene expression levels. These findings provided novel insights into the gut microbiota and transcriptomic responses of mandarin fish to different dietary types.
鳜鱼（Siniperca chuatsi）是中国典型的肉食性淡水经济鱼类。近年来，研究其对配合饲料的摄食行为适应性已成为一个研究重点。本研究评估了不同饲料对鳜鱼体成分、营养成分、消化酶活性、基因表达和肠道微生物群的影响。首先，在活饵组（LFSC）和配合饲料组（CFSC）之间，肌肉的基本营养成分（水分、粗蛋白、粗脂肪和粗灰分）以及脂肪酸和氨基酸含量均未发现显著差异。然而，与CFSC组相比，LFSC组鳜鱼肝脏和肠道中的脂肪酶活性显著更高，而肠道中的淀粉酶活性则呈现相反的模式。此外，肠道转录组分析揭示了6238个差异表达基因，并鉴定了几个与饲料类型相关的差异表达生物钟基因。此外，肠道微生物群分析表明，不同的喂养方式会影响微生物组成，揭示了细菌属与肠道基因表达水平之间的相关性。这些发现为鳜鱼肠道微生物群及其对不同饮食类型的转录组反应提供了新的见解。
