College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei, 430070, China.
Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, 430070, China.
Funct Integr Genomics. 2024 Oct 25;24(6):197. doi: 10.1007/s10142-024-01471-3.
The mandarin fish (Siniperca chuatsi), as a typical freshwater carnivorous fish, has high economic value. Mandarin fish have a peculiar feeding habit of feeding on other live fry during the first-feeding period, while rejecting zooplankton or particulate feed, which may be attributed to the low expression of zooplankton-associated gene sws1 in mandarin fish. The domesticated strain of mandarin fish could feed on Artemia at 3 days post hatching (dph). However, the mechanism of mandarin fish larvae recognize and forage Artemia as food is still unclear. In this study, we employed transcriptional analysis to identify the representative differential pathways between mandarin fish larvae unfed and fed with Artemia at 3 dph. The comparative transcriptome analysis has unveiled a tapestry of genetic expression, highlighting 403 genes that have been up-regulated and 259 that have been down-regulated, all of which constitute the differentially expressed genes (DEGs). KEGG pathway analysis revealed that the number of differentially expressed genes in the photoconductive signaling pathway was the largest. Next, the Vorinostat (suberoylanilide hydroxamic acid, SAHA) was used to assess whether sws1 induced ingestion of Artemia in mandarin fish larvae. We discovered that SAHA-treated larvae had more food intake of Artemia and up-regulated the transcription level of npy, which might have been associated with the up-regulated of sws1 opsin. Additionally, exposure to 0.5 µM SAHA increased the expression of genes involved in phototransduction pathway. These findings would provide insights on the molecular processes involved in mandarin fish larvae feeding on Artemia at the first-feeding stage.
鳜鱼（Siniperca chuatsi）作为一种典型的淡水肉食性鱼类，具有很高的经济价值。鳜鱼在首次摄食期间有一个奇特的摄食习惯，即摄食其他活鱼苗，而排斥浮游动物或颗粒饲料，这可能是由于鳜鱼中与浮游动物相关的基因 sws1 表达较低。经过驯化的鳜鱼苗在孵化后 3 天（dph）就可以摄食卤虫。然而，鳜鱼幼鱼识别和觅食卤虫的机制尚不清楚。在本研究中，我们采用转录组分析方法鉴定了鳜鱼幼鱼未摄食和摄食卤虫 3 dph 的代表性差异途径。比较转录组分析揭示了遗传表达的多样性，突出了 403 个上调基因和 259 个下调基因，它们构成了差异表达基因（DEGs）。KEGG 通路分析表明，光导信号通路中差异表达基因的数量最多。接下来，使用伏立诺他（丁酸钠，SAHA）来评估 sws1 是否诱导鳜鱼幼鱼摄食卤虫。我们发现，SAHA 处理的幼鱼对卤虫的摄食量增加，并且 npy 的转录水平上调，这可能与 sws1 视蛋白的上调有关。此外，暴露于 0.5 µM SAHA 增加了光转导途径中参与的基因的表达。这些发现将为鳜鱼幼鱼在首次摄食阶段摄食卤虫的分子过程提供新的见解。
