Department of Basic Sciences, Division of Anatomy and Embryology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran; Stem Cells and Transgenic Technology Research Center (STTRC), Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Basic Sciences, Division of Anatomy and Embryology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Reprod Toxicol. 2024 Oct;129:108688. doi: 10.1016/j.reprotox.2024.108688. Epub 2024 Aug 12.
Formaldehyde (FA) as a common organic compound has been shown to cause placental dysfunction and fetal defects. The potential benefits of fish oil (FOil) in protecting placental structures are attributed to its antioxidant properties. This study aimed to explore the preventive role of FOil in mitigating the adverse effects of FA in pregnant rats. Thirty pregnant Wistar rats were randomly categorized into five groups of control, sham (Normal saline; Orally and intraperitoneally), FOil (0.5 ml/day; Orally), FA (5 mg/kg/bw; intraperitoneally), FA+FOil. The treatment period was from day 0-20 of pregnancy. On the 20th day of pregnancy, placental morphometric parameters were measured. The histological and histochemical analyses were performed using H&E and PAS staining, respectively. Also, the placenta tissue was analyzed for oxidative stress biomarkers, p-53 protein levels, and the expression of caspase-3 gene. The administration of FA led to a significant decrease in the weight, diameter, and thickness of the placenta, as well as a decrease in the thickness of the decidua layer, junctional and labyrinth zone, and the number of trophoblast giant cells in rat placentas. FA led to a significant increase in placental p-53 protein levels, caspase-3 expression, and oxidative stress biomarkers. Administration of FOil to pregnant rats treated with FA led to a significant decrease in morphometric and histological changes, oxidative stress, and the expression of genes associated with apoptosis. The findings suggest that the administration of FOil to FA-treated pregnant rats can protect placental histopathological changes by enhancing the activity of the antioxidant enzymes.
甲醛（FA）作为一种常见的有机化合物，已被证明会导致胎盘功能障碍和胎儿缺陷。鱼油（FOil）具有抗氧化特性，其保护胎盘结构的潜在益处归因于此。本研究旨在探讨 FOil 在减轻 FA 对怀孕大鼠的不良影响方面的预防作用。将 30 只怀孕的 Wistar 大鼠随机分为五组：对照组、假手术组（生理盐水；口服和腹腔内注射）、FOil 组（0.5ml/天；口服）、FA 组（5mg/kg/bw；腹腔内注射）、FA+FOil 组。治疗期为妊娠第 0-20 天。在妊娠第 20 天测量胎盘形态计量参数。使用 H&E 和 PAS 染色分别进行组织学和组织化学分析。此外，还分析了胎盘组织中的氧化应激生物标志物、p-53 蛋白水平和 caspase-3 基因的表达。FA 的给药导致大鼠胎盘的重量、直径和厚度显著降低，蜕膜层、连接和迷路区的厚度以及滋养层巨细胞的数量减少。FA 导致胎盘 p-53 蛋白水平、caspase-3 表达和氧化应激生物标志物显著增加。向接受 FA 处理的怀孕大鼠给予 FOil 可显著减少形态和组织学变化、氧化应激以及与细胞凋亡相关的基因表达。这些发现表明，向接受 FA 处理的怀孕大鼠给予 FOil 可通过增强抗氧化酶的活性来保护胎盘的组织病理学变化。
