School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou 510182, China.
Department of Chemical and Environmental Engineering, Hetao College, Bayannaoer 015000, China.
Int J Biol Macromol. 2024 Oct;278(Pt 1):134686. doi: 10.1016/j.ijbiomac.2024.134686. Epub 2024 Aug 12.
This study presents a thrombin-loaded cationized chitosan (TCCS) sponge with highly effective hemostatic and antibacterial activity. The TCCS sponge, prepared using a multistep method, features a porous structure, favorable mechanical properties, excellent water absorption ability, and shape recovery triggered by water or blood. The TCCS sponge exhibited strong antibacterial activity against Methicillin-resistant Staphylococcus aureus and Escherichia coli. Additionally, it demonstrated enhanced procoagulant and hemostatic efficacy in rat tail amputation and rat liver perforation wound models compared to commercial hemostats. Furthermore, the sponge exhibited favorable biocompatibility and biosafety. These findings suggest that the TCCS sponge has substantial potential for practical applications in managing severe hemorrhages and bacterial infections.
本研究提出了一种载有凝血酶的阳离子化壳聚糖（TCCS）海绵，具有高效的止血和抗菌活性。TCCS 海绵采用多步法制得，具有多孔结构、良好的机械性能、优异的吸水性以及水或血液触发的形状回复能力。TCCS 海绵对耐甲氧西林金黄色葡萄球菌和大肠杆菌具有强烈的抗菌活性。此外，与商业止血剂相比，它在大鼠尾切断和大鼠肝穿孔伤口模型中表现出增强的促凝和止血效果。此外，该海绵还表现出良好的生物相容性和生物安全性。这些发现表明 TCCS 海绵在处理严重出血和细菌感染方面具有很大的应用潜力。
