Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering. Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering. Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Anal Chim Acta. 2024 Sep 1;1320:343015. doi: 10.1016/j.aca.2024.343015. Epub 2024 Jul 23.
Excessive use of antibiotics will enter the water environment and soil through the biological chain, and then transfer to the human body through food, resulting in drug resistance, kidney toxicity and other health problems, so it is urgent to develop highly sensitive detection methods of antibiotics. Here, we designed a dual-mode sensor platform based on closed bipolar electrode (cBPE) electroluminescence (ECL) and mobile phone imaging to detect kanamycin in seawater. The prepared CN-NV-550 displayed extremely intense ECL signal, allowing for convenient mobile phone imaging. The cBPE was combined with DNA cycle amplification technology to prevent the mutual interference between target and the luminescent material, and realized the amplification of signal. In the presence of target Kana, CoO was introduced to the cBPE anode by DNA cycle amplification product, and accelerated the oxidation rate of uric acid (UA). Thus, the electroluminescence response of CN-NV-550 on cBPE cathode was much improved due to the charge balance of the cBPE, achieving both ECL detection and mobile phone imaging assay of Kana, which much improved the accuracy and efficiency of assay. The limit of detection (LOD) in this work is 0.23 pM, and LOD for mobile phone imaging is 0.39 pM. This study integrate ECL imaging visualization of CN-NV-550 and high electrocatalytic activity of CoO into cBPE-ECL detection, providing a new perspective for antibiotic analysis, and has great potential for practical applications, especially in Marine environmental pollution monitoring.
过度使用抗生素会通过生物链进入水环境和土壤，然后通过食物转移到人体，导致抗药性、肾毒性等健康问题，因此迫切需要开发高灵敏度的抗生素检测方法。在这里，我们设计了一种基于闭双极电极（cBPE）电致发光（ECL）和手机成像的双模传感器平台，用于检测海水中的卡那霉素。所制备的 CN-NV-550 显示出极其强烈的 ECL 信号，便于手机成像。cBPE 与 DNA 循环扩增技术相结合，可防止目标物与发光材料之间的相互干扰，并实现信号的放大。在存在靶标 Kana 的情况下，CoO 通过 DNA 循环扩增产物被引入到 cBPE 阳极，从而加速尿酸（UA）的氧化速率。因此，由于 cBPE 的电荷平衡，CN-NV-550 在 cBPE 阴极上的 ECL 响应大大提高，实现了 Kana 的 ECL 检测和手机成像检测，大大提高了检测的准确性和效率。在这项工作中，检测限（LOD）为 0.23 pM，手机成像的 LOD 为 0.39 pM。本研究将 CN-NV-550 的 ECL 成像可视化和 CoO 的高电催化活性集成到 cBPE-ECL 检测中，为抗生素分析提供了新的视角，具有很大的实际应用潜力，特别是在海洋环境污染监测方面。
